Ontogeny of T cells, B cells and monocytes in the bovine foetus

D. R. Senogles, P. S. Paul, D. W. Johnson, C. C. Muscoplat

Research output: Contribution to journalArticle

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Abstract

The ontogeny of lymphocyte subpopulations and monocytes was studied in developing bovine foetuses. Fifty-four bovine foetuses ranging in age from 3 to 9 months of gestation were collected and examined for the presence of thymus-derived (T lymphocytes), bone marrow-derived (B lymphocytes) and phagocytic (macrophage) cells from thymus, spleen and peripheral blood. Detection of the cells expressing T lymphocyte surface markers was done using a modified sheep red blood cell rosette assay. Lymphocytes bearing surface immunoglobulin (sIg) were detected by staining with a fluoresceinated rabbit antibody prepared against bovine IgG. The procedure used for detecting monocytes was a latex bead phagocytic assay. In the thymus, the percentage of T lymphocytes was relatively constant throughout gestation at approximately 60-70%, with B lymphocytes remaining at about 1%, whereas the phagocytic cells showed an increase from 1% at 3 months to approximately 8% at term. The spleen showed an increasing percentage of T lymphocytes, from 11% at 3 months to over 40% at term, whereas the percentage of B lymphocytes remained consistent at about 2-3% from 3 months until term. The percentage of monocytes increases from 1% at 3 months to over 20% at 9 months. Peripheral blood T lymphocytes increased steadily from approximately 1% at 3 months to 45% at term. B lymphocyte levels remained low, about 1%, throughout gestation, whereas monocytes increased from 1% at 3 months to 5% at 9 months.

Original languageEnglish (US)
Pages (from-to)299-303
Number of pages5
JournalClinical and Experimental Immunology
Volume36
Issue number2
StatePublished - Jan 1 1979

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Monocytes
Fetus
B-Lymphocytes
T-Lymphocytes
Thymus Gland
Phagocytes
Pregnancy
Spleen
B-Cell Antigen Receptors
Lymphocyte Subsets
Microspheres
Sheep
Immunoglobulin G
Erythrocytes
Bone Marrow
Macrophages
Lymphocytes
Staining and Labeling
Rabbits
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Senogles, D. R., Paul, P. S., Johnson, D. W., & Muscoplat, C. C. (1979). Ontogeny of T cells, B cells and monocytes in the bovine foetus. Clinical and Experimental Immunology, 36(2), 299-303.

Ontogeny of T cells, B cells and monocytes in the bovine foetus. / Senogles, D. R.; Paul, P. S.; Johnson, D. W.; Muscoplat, C. C.

In: Clinical and Experimental Immunology, Vol. 36, No. 2, 01.01.1979, p. 299-303.

Research output: Contribution to journalArticle

Senogles, DR, Paul, PS, Johnson, DW & Muscoplat, CC 1979, 'Ontogeny of T cells, B cells and monocytes in the bovine foetus', Clinical and Experimental Immunology, vol. 36, no. 2, pp. 299-303.
Senogles DR, Paul PS, Johnson DW, Muscoplat CC. Ontogeny of T cells, B cells and monocytes in the bovine foetus. Clinical and Experimental Immunology. 1979 Jan 1;36(2):299-303.
Senogles, D. R. ; Paul, P. S. ; Johnson, D. W. ; Muscoplat, C. C. / Ontogeny of T cells, B cells and monocytes in the bovine foetus. In: Clinical and Experimental Immunology. 1979 ; Vol. 36, No. 2. pp. 299-303.
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