On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis-fluorescence line narrowing spectroscopy

Kenneth P. Roberts, Cheng H. Lin, Maria Singhal, George P Casale, Gerald J. Small, Ryszard Jankowiak

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The benzo[a]pyrene (BP)-derived 7-(benzo[a]pyren-6-yl)guanine (BP-6- N7Gua) depurinating one-electron oxidation adduct was identified in the urine extracts of coal-smoke-exposed humans for the first time. Urine samples were prepared by solid-phase extraction and reversed-phase high-performance liquid chromatography. Subsequently, the BP-6-N7Gua adduct was identified on-line with capillary electrophoresis fluorescence line narrowing spectroscopy (CE- FLNS) at 4.2 K. The daily excretion of BP-6-N7Gua in human urine of individuals exposed to coal smoke was approximately 226 pmol per μmol of creatinine. Due to the high level of excretion we propose that BP-6-N7Gua adducts found in urine could serve as effective biomarkers for risk assessment of BP exposure. The results demonstrate that CE-FLNS allows for on-line separation and DNA adducts identification in complex fluid extracts.

Original languageEnglish (US)
Pages (from-to)799-806
Number of pages8
JournalELECTROPHORESIS
Volume21
Issue number4
DOIs
StatePublished - Apr 3 2000

Fingerprint

Capillary electrophoresis
DNA Adducts
Capillary Electrophoresis
Spectrum Analysis
Fluorescence
Urine
Spectroscopy
Coal
Smoke
Benzo(a)pyrene
Solid Phase Extraction
High performance liquid chromatography
Biomarkers
Reverse-Phase Chromatography
Complex Mixtures
Risk assessment
Creatinine
High Pressure Liquid Chromatography
7-(benzo(a)pyren-6-yl)guanine
Electrons

Keywords

  • Analyte identification
  • Capillary electrophoresis
  • Depurinating DNA adducts
  • Fluorescence line narrowing
  • One-electron oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis-fluorescence line narrowing spectroscopy. / Roberts, Kenneth P.; Lin, Cheng H.; Singhal, Maria; Casale, George P; Small, Gerald J.; Jankowiak, Ryszard.

In: ELECTROPHORESIS, Vol. 21, No. 4, 03.04.2000, p. 799-806.

Research output: Contribution to journalArticle

Roberts, Kenneth P. ; Lin, Cheng H. ; Singhal, Maria ; Casale, George P ; Small, Gerald J. ; Jankowiak, Ryszard. / On-line identification of depurinating DNA adducts in human urine by capillary electrophoresis-fluorescence line narrowing spectroscopy. In: ELECTROPHORESIS. 2000 ; Vol. 21, No. 4. pp. 799-806.
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