ON and off retinal ganglion cells differentially regulate serotonergic and GABAergic activity in the dorsal raphe nucleus

Ting Zhang, Lu Huang, Li Zhang, Minjie Tan, Mingliang Pu, Gary E Pickard, Kwok Fai So, Chaoran Ren

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The dorsal raphe nucleus (DRN), the major source of serotonergic input to the forebrain, receives excitatory input from the retina that can modulate serotonin levels and depressive-like behavior. In the Mongolian gerbil, retinal ganglion cells (RGCs) with alpha-like morphological and Y-like physiological properties innervate the DRN with ON DRN-projecting RGCs out numbering OFF DRN-projecting RGCs. The DRN neurons targeted by ON and OFF RGCs are unknown. To explore retino-raphe anatomical organization, retinal afferents labeled with Cholera toxin B were examined for association with the postsynaptic protein PSD-95. Synaptic associations between retinal afferents and DRN serotonergic and GABAergic neurons were observed. To explore retino-raphe functional organization, light-evoked c-fos expression was examined. Light significantly increased the number of DRN serotonergic and GABAergic cells expressing c-Fos. When ON RGCs were rendered silent while enhancing the firing rate of OFF RGCs, c-Fos expression was greatly increased in DRN serotonergic neurons suggesting that OFF DRN-projecting RGCs predominately activate serotonergic neurons whereas ON DRN-projecting RGCs mainly target GABAergic neurons. Direct glutamatergic retinal input to DRN 5-HT neurons contributes to the complex excitatory drive regulating these cells. Light, via the retinoraphe pathway can modify DRN 5-HT neuron activity which may play a role in modulating affective behavior.

Original languageEnglish (US)
Article number26060
JournalScientific Reports
Volume6
DOIs
StatePublished - May 16 2016

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Retinal Ganglion Cells
Serotonergic Neurons
GABAergic Neurons
Serotonin
Neurons
Light
Dorsal Raphe Nucleus
Gerbillinae
Cholera Toxin
Prosencephalon
Retina

ASJC Scopus subject areas

  • General

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ON and off retinal ganglion cells differentially regulate serotonergic and GABAergic activity in the dorsal raphe nucleus. / Zhang, Ting; Huang, Lu; Zhang, Li; Tan, Minjie; Pu, Mingliang; Pickard, Gary E; So, Kwok Fai; Ren, Chaoran.

In: Scientific Reports, Vol. 6, 26060, 16.05.2016.

Research output: Contribution to journalArticle

Zhang, Ting ; Huang, Lu ; Zhang, Li ; Tan, Minjie ; Pu, Mingliang ; Pickard, Gary E ; So, Kwok Fai ; Ren, Chaoran. / ON and off retinal ganglion cells differentially regulate serotonergic and GABAergic activity in the dorsal raphe nucleus. In: Scientific Reports. 2016 ; Vol. 6.
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abstract = "The dorsal raphe nucleus (DRN), the major source of serotonergic input to the forebrain, receives excitatory input from the retina that can modulate serotonin levels and depressive-like behavior. In the Mongolian gerbil, retinal ganglion cells (RGCs) with alpha-like morphological and Y-like physiological properties innervate the DRN with ON DRN-projecting RGCs out numbering OFF DRN-projecting RGCs. The DRN neurons targeted by ON and OFF RGCs are unknown. To explore retino-raphe anatomical organization, retinal afferents labeled with Cholera toxin B were examined for association with the postsynaptic protein PSD-95. Synaptic associations between retinal afferents and DRN serotonergic and GABAergic neurons were observed. To explore retino-raphe functional organization, light-evoked c-fos expression was examined. Light significantly increased the number of DRN serotonergic and GABAergic cells expressing c-Fos. When ON RGCs were rendered silent while enhancing the firing rate of OFF RGCs, c-Fos expression was greatly increased in DRN serotonergic neurons suggesting that OFF DRN-projecting RGCs predominately activate serotonergic neurons whereas ON DRN-projecting RGCs mainly target GABAergic neurons. Direct glutamatergic retinal input to DRN 5-HT neurons contributes to the complex excitatory drive regulating these cells. Light, via the retinoraphe pathway can modify DRN 5-HT neuron activity which may play a role in modulating affective behavior.",
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