OMA-AML-1

A leukemic myeloid cell line with CD34+ progenitor and CD15+ spontaneously differentiating cell compartments

Samuel Jay Pirruccello, John D. Jackson, Molly S. Lang, Joanne DeBoer, Sally Mann, David Crouse, William P. Vaughan, Karel A. Dicke, John G Sharp

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OMA-AML-1 was established from a patient with acute myelomonocytic (M4) leukemia at fifth relapse when blasts were greater than 85% CD34+, CD15-. Leukemic cells were established in suspension culture and independently grown as subcutaneous tumors in SCID mice. Cells growing in suspension culture underwent differentiation by phenotypic and morphologic criteria. In contrast, cells grown as subcutaneous solid tumors in SCID mice maintained progenitor cell characteristics with high-density CD34 expression and lack of morphologic differentiation. A tendency toward differentiation to CD15+, CD34- cells in vitro and self-renewal of CD34+, CD15- cells in vivo was consistently demonstrated regardless of whether cells were initially grown in vitro or in vivo. The cell line maintains both a CD34+, CD15- progenitor cell pool and a non-overlapping, CD15+, CD34- differentiating cell compartment after more than 1 year in continuous culture. Cell cycle analysis and cloning experiments were consistent with terminal differentiation occurring in the CD15+, CD34- population. The cell line shows concentration-dependent proliferative responses to interleukin (IL)-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-6, but not to granulocyte CSF (G-CSF). OMA-AML-1 appears to mimic several features of normal myeloid hematopoiesis and should prove useful for the study of normal and malignant myeloid differentiation.

Original languageEnglish (US)
Pages (from-to)1026-1032
Number of pages7
JournalBlood
Volume80
Issue number4
StatePublished - Jan 1 1992

Fingerprint

Myeloid Cells
Cells
Cell Line
Tumors
Suspensions
SCID Mice
Interleukin-3
Cloning
Granulocyte-Macrophage Colony-Stimulating Factor
Cell culture
Stem Cells
Interleukin-6
Leukemia, Myelomonocytic, Acute
Hematopoiesis
Granulocytes
Organism Cloning
Neoplasms
Cell Cycle
Experiments
Recurrence

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

OMA-AML-1 : A leukemic myeloid cell line with CD34+ progenitor and CD15+ spontaneously differentiating cell compartments. / Pirruccello, Samuel Jay; Jackson, John D.; Lang, Molly S.; DeBoer, Joanne; Mann, Sally; Crouse, David; Vaughan, William P.; Dicke, Karel A.; Sharp, John G.

In: Blood, Vol. 80, No. 4, 01.01.1992, p. 1026-1032.

Research output: Contribution to journalArticle

Pirruccello, SJ, Jackson, JD, Lang, MS, DeBoer, J, Mann, S, Crouse, D, Vaughan, WP, Dicke, KA & Sharp, JG 1992, 'OMA-AML-1: A leukemic myeloid cell line with CD34+ progenitor and CD15+ spontaneously differentiating cell compartments', Blood, vol. 80, no. 4, pp. 1026-1032.
Pirruccello, Samuel Jay ; Jackson, John D. ; Lang, Molly S. ; DeBoer, Joanne ; Mann, Sally ; Crouse, David ; Vaughan, William P. ; Dicke, Karel A. ; Sharp, John G. / OMA-AML-1 : A leukemic myeloid cell line with CD34+ progenitor and CD15+ spontaneously differentiating cell compartments. In: Blood. 1992 ; Vol. 80, No. 4. pp. 1026-1032.
@article{05c8efdcd1bc483087f6c6ea66ab140e,
title = "OMA-AML-1: A leukemic myeloid cell line with CD34+ progenitor and CD15+ spontaneously differentiating cell compartments",
abstract = "OMA-AML-1 was established from a patient with acute myelomonocytic (M4) leukemia at fifth relapse when blasts were greater than 85{\%} CD34+, CD15-. Leukemic cells were established in suspension culture and independently grown as subcutaneous tumors in SCID mice. Cells growing in suspension culture underwent differentiation by phenotypic and morphologic criteria. In contrast, cells grown as subcutaneous solid tumors in SCID mice maintained progenitor cell characteristics with high-density CD34 expression and lack of morphologic differentiation. A tendency toward differentiation to CD15+, CD34- cells in vitro and self-renewal of CD34+, CD15- cells in vivo was consistently demonstrated regardless of whether cells were initially grown in vitro or in vivo. The cell line maintains both a CD34+, CD15- progenitor cell pool and a non-overlapping, CD15+, CD34- differentiating cell compartment after more than 1 year in continuous culture. Cell cycle analysis and cloning experiments were consistent with terminal differentiation occurring in the CD15+, CD34- population. The cell line shows concentration-dependent proliferative responses to interleukin (IL)-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-6, but not to granulocyte CSF (G-CSF). OMA-AML-1 appears to mimic several features of normal myeloid hematopoiesis and should prove useful for the study of normal and malignant myeloid differentiation.",
author = "Pirruccello, {Samuel Jay} and Jackson, {John D.} and Lang, {Molly S.} and Joanne DeBoer and Sally Mann and David Crouse and Vaughan, {William P.} and Dicke, {Karel A.} and Sharp, {John G}",
year = "1992",
month = "1",
day = "1",
language = "English (US)",
volume = "80",
pages = "1026--1032",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "4",

}

TY - JOUR

T1 - OMA-AML-1

T2 - A leukemic myeloid cell line with CD34+ progenitor and CD15+ spontaneously differentiating cell compartments

AU - Pirruccello, Samuel Jay

AU - Jackson, John D.

AU - Lang, Molly S.

AU - DeBoer, Joanne

AU - Mann, Sally

AU - Crouse, David

AU - Vaughan, William P.

AU - Dicke, Karel A.

AU - Sharp, John G

PY - 1992/1/1

Y1 - 1992/1/1

N2 - OMA-AML-1 was established from a patient with acute myelomonocytic (M4) leukemia at fifth relapse when blasts were greater than 85% CD34+, CD15-. Leukemic cells were established in suspension culture and independently grown as subcutaneous tumors in SCID mice. Cells growing in suspension culture underwent differentiation by phenotypic and morphologic criteria. In contrast, cells grown as subcutaneous solid tumors in SCID mice maintained progenitor cell characteristics with high-density CD34 expression and lack of morphologic differentiation. A tendency toward differentiation to CD15+, CD34- cells in vitro and self-renewal of CD34+, CD15- cells in vivo was consistently demonstrated regardless of whether cells were initially grown in vitro or in vivo. The cell line maintains both a CD34+, CD15- progenitor cell pool and a non-overlapping, CD15+, CD34- differentiating cell compartment after more than 1 year in continuous culture. Cell cycle analysis and cloning experiments were consistent with terminal differentiation occurring in the CD15+, CD34- population. The cell line shows concentration-dependent proliferative responses to interleukin (IL)-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-6, but not to granulocyte CSF (G-CSF). OMA-AML-1 appears to mimic several features of normal myeloid hematopoiesis and should prove useful for the study of normal and malignant myeloid differentiation.

AB - OMA-AML-1 was established from a patient with acute myelomonocytic (M4) leukemia at fifth relapse when blasts were greater than 85% CD34+, CD15-. Leukemic cells were established in suspension culture and independently grown as subcutaneous tumors in SCID mice. Cells growing in suspension culture underwent differentiation by phenotypic and morphologic criteria. In contrast, cells grown as subcutaneous solid tumors in SCID mice maintained progenitor cell characteristics with high-density CD34 expression and lack of morphologic differentiation. A tendency toward differentiation to CD15+, CD34- cells in vitro and self-renewal of CD34+, CD15- cells in vivo was consistently demonstrated regardless of whether cells were initially grown in vitro or in vivo. The cell line maintains both a CD34+, CD15- progenitor cell pool and a non-overlapping, CD15+, CD34- differentiating cell compartment after more than 1 year in continuous culture. Cell cycle analysis and cloning experiments were consistent with terminal differentiation occurring in the CD15+, CD34- population. The cell line shows concentration-dependent proliferative responses to interleukin (IL)-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-6, but not to granulocyte CSF (G-CSF). OMA-AML-1 appears to mimic several features of normal myeloid hematopoiesis and should prove useful for the study of normal and malignant myeloid differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0026742453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026742453&partnerID=8YFLogxK

M3 - Article

VL - 80

SP - 1026

EP - 1032

JO - Blood

JF - Blood

SN - 0006-4971

IS - 4

ER -