Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes

David J. Gonzalez, Yuquan Xu, Yu Liang Yang, Eduardo Esquenazi, Wei Ting Liu, Anna Edlund, Tram Duong, Liangcheng Du, István Molnár, William H. Gerwick, Paul R. Jensen, Michael Fischbach, Chih Chuang Liaw, Paul Straight, Victor Nizet, Pieter C. Dorrestein

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Many microbes can be cultured as single-species communities. Often, these colonies are controlled and maintained via the secretion of metabolites. Such metabolites have been an invaluable resource for the discovery of therapeutics (e.g. penicillin, taxol, rapamycin, epothilone). In this article, written for a special issue on imaging mass spectrometry, we show that MALDI-imaging mass spectrometry can be adapted to observe, in a spatial manner, the metabolic exchange patterns of a diverse array of microbes, including thermophilic and mesophilic fungi, cyanobacteria, marine and terrestrial actinobacteria, and pathogenic bacteria. Dependent on media conditions, on average and based on manual analysis, we observed 11.3 molecules associated with each microbial IMS experiment, which was split nearly 50:50 between secreted and colony-associated molecules. The spatial distributions of these metabolic exchange factors are related to the biological and ecological functions of the organisms. This work establishes that MALDI-based IMS can be used as a general tool to study a diverse array of microbes. Furthermore the article forwards the notion of the IMS platform as a window to discover previously unreported molecules by monitoring the metabolic exchange patterns of organisms when grown on agar substrates.This article is part of a Special Issue entitled: Imaging Mass Spectrometry: A User's Guide to a New Technique for Biological and Biomedical Research.

Original languageEnglish (US)
Pages (from-to)5069-5076
Number of pages8
JournalJournal of Proteomics
Volume75
Issue number16
DOIs
StatePublished - Aug 30 2012

Fingerprint

Mass spectrometry
Mass Spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Metabolites
Imaging techniques
Molecules
Epothilones
Actinobacteria
Cyanobacteria
Sirolimus
Paclitaxel
Fungi
Penicillins
Spatial distribution
Agar
Biomedical Research
Bacteria
Monitoring
Substrates
Experiments

Keywords

  • Metabolic exchange
  • Microbial ecology
  • Natural products
  • Virulence factors

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Gonzalez, D. J., Xu, Y., Yang, Y. L., Esquenazi, E., Liu, W. T., Edlund, A., ... Dorrestein, P. C. (2012). Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes. Journal of Proteomics, 75(16), 5069-5076. https://doi.org/10.1016/j.jprot.2012.05.036

Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes. / Gonzalez, David J.; Xu, Yuquan; Yang, Yu Liang; Esquenazi, Eduardo; Liu, Wei Ting; Edlund, Anna; Duong, Tram; Du, Liangcheng; Molnár, István; Gerwick, William H.; Jensen, Paul R.; Fischbach, Michael; Liaw, Chih Chuang; Straight, Paul; Nizet, Victor; Dorrestein, Pieter C.

In: Journal of Proteomics, Vol. 75, No. 16, 30.08.2012, p. 5069-5076.

Research output: Contribution to journalArticle

Gonzalez, DJ, Xu, Y, Yang, YL, Esquenazi, E, Liu, WT, Edlund, A, Duong, T, Du, L, Molnár, I, Gerwick, WH, Jensen, PR, Fischbach, M, Liaw, CC, Straight, P, Nizet, V & Dorrestein, PC 2012, 'Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes', Journal of Proteomics, vol. 75, no. 16, pp. 5069-5076. https://doi.org/10.1016/j.jprot.2012.05.036
Gonzalez, David J. ; Xu, Yuquan ; Yang, Yu Liang ; Esquenazi, Eduardo ; Liu, Wei Ting ; Edlund, Anna ; Duong, Tram ; Du, Liangcheng ; Molnár, István ; Gerwick, William H. ; Jensen, Paul R. ; Fischbach, Michael ; Liaw, Chih Chuang ; Straight, Paul ; Nizet, Victor ; Dorrestein, Pieter C. / Observing the invisible through imaging mass spectrometry, a window into the metabolic exchange patterns of microbes. In: Journal of Proteomics. 2012 ; Vol. 75, No. 16. pp. 5069-5076.
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