O-linkage of N-acetylglucosamine to Sp1 activation domain inhibits its transcriptional capability

Xiaoyong Yang, Kaihong Su, Mark D. Roos, Qing Chang, Andrew J. Paterson, Jeffrey E. Kudlow

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

The posttranslational modification of eukaryotic intracellular proteins by O-linked N-acetylglucosamine (O-GlcNAc) monosaccharides is essential for cell viability, yet its precise functional roles are largely unknown. O-GlcNAc transferase utilizes UDP-GlcNAc, the end product of hexosamine biosynthesis, to catalyze this modification. The availability of UDP-GlcNAc correlates with glycosylation levels of intracellular proteins as well as with transcriptional levels of some genes. Meanwhile, transcription factors and RNA polymerase II can be modified by O-GlcNAc. A linkage between transcription factor O-GlcNAcylation and transcriptional regulation therefore has been postulated. Here, we show that O-GlcNAcylation of a chimeric transcriptional activator containing the second activation domain of Sp1 decreases its transcriptional activity both in an in vitro transcription system and in living cells, which is in concert with our observation that O-GlcNAcylation of Sp1 activation domain blocks its in vitro and in vivo interactions with other Sp1 molecules and TATA-binding protein-associated factor II 110. Furthermore, overexpression of O-GlcNAc transferase specifically inhibits transcriptional activation by native Sp1 in cells. Thus, our studies provide direct evidence that O-GlcNAcylation of transcription factors is involved in transcriptional regulation.

Original languageEnglish (US)
Pages (from-to)6611-6616
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number12
DOIs
StatePublished - Jun 5 2001

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Acetylglucosamine
Uridine Diphosphate
Transcription Factors
TATA-Binding Protein Associated Factors
Hexosamines
Monosaccharides
RNA Polymerase II
Post Translational Protein Processing
Glycosylation
Transcriptional Activation
Cell Survival
Proteins
Genes
O-GlcNAc transferase
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

O-linkage of N-acetylglucosamine to Sp1 activation domain inhibits its transcriptional capability. / Yang, Xiaoyong; Su, Kaihong; Roos, Mark D.; Chang, Qing; Paterson, Andrew J.; Kudlow, Jeffrey E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 12, 05.06.2001, p. 6611-6616.

Research output: Contribution to journalArticle

Yang, Xiaoyong ; Su, Kaihong ; Roos, Mark D. ; Chang, Qing ; Paterson, Andrew J. ; Kudlow, Jeffrey E. / O-linkage of N-acetylglucosamine to Sp1 activation domain inhibits its transcriptional capability. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 12. pp. 6611-6616.
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