Nutritional control of dimorphism in Ceratocystis ulmi

Rajiv K. Kulkarni, Kenneth W. Nickerson

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The nutritional parameters controlling the yeast-mycelial dimorphism in Ceratocystis ulmi, the causative agent of Dutch elm disease, have been established. The nitrogen source is critical. In a defined glucose-salts liquid medium the presence of proline induces the yeast morphology whereas ammonium, arginine, or asparagine induces the mycelial state. This phenomenon is not a secondary manifestation of pH changes in the respective media and it can be achieved with either a blastospore or a conidiospore inoculum. The dimorphism is, however, dependent on the inoculum size. Yeasts are only formed in the proline-containing medium with inocula giving cell concentrations of ≥106 blastospores/ml. Once the spores produce visible buds or germ tubes they are "committed" to that developmental pattern in the sense that if they are now resuspended in the opposite medium, at the same cell concentration, the alternate morphology can no longer be produced.

Original languageEnglish (US)
Pages (from-to)148-154
Number of pages7
JournalExperimental Mycology
Volume5
Issue number2
DOIs
StatePublished - Jun 1981

Fingerprint

Ulmus
Yeasts
Proline
Asparagine
Spores
Ammonium Compounds
Arginine
Nitrogen
Salts
Glucose

Keywords

  • Ceratocystis ulmi
  • Dutch elm disease
  • fungal nitrogen metabolism
  • yeast-mycelial dimorphism

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Nutritional control of dimorphism in Ceratocystis ulmi. / Kulkarni, Rajiv K.; Nickerson, Kenneth W.

In: Experimental Mycology, Vol. 5, No. 2, 06.1981, p. 148-154.

Research output: Contribution to journalArticle

@article{eef5b838751847ab86ec861f7f87029f,
title = "Nutritional control of dimorphism in Ceratocystis ulmi",
abstract = "The nutritional parameters controlling the yeast-mycelial dimorphism in Ceratocystis ulmi, the causative agent of Dutch elm disease, have been established. The nitrogen source is critical. In a defined glucose-salts liquid medium the presence of proline induces the yeast morphology whereas ammonium, arginine, or asparagine induces the mycelial state. This phenomenon is not a secondary manifestation of pH changes in the respective media and it can be achieved with either a blastospore or a conidiospore inoculum. The dimorphism is, however, dependent on the inoculum size. Yeasts are only formed in the proline-containing medium with inocula giving cell concentrations of ≥106 blastospores/ml. Once the spores produce visible buds or germ tubes they are {"}committed{"} to that developmental pattern in the sense that if they are now resuspended in the opposite medium, at the same cell concentration, the alternate morphology can no longer be produced.",
keywords = "Ceratocystis ulmi, Dutch elm disease, fungal nitrogen metabolism, yeast-mycelial dimorphism",
author = "Kulkarni, {Rajiv K.} and Nickerson, {Kenneth W.}",
year = "1981",
month = "6",
doi = "10.1016/0147-5975(81)90015-3",
language = "English (US)",
volume = "5",
pages = "148--154",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Nutritional control of dimorphism in Ceratocystis ulmi

AU - Kulkarni, Rajiv K.

AU - Nickerson, Kenneth W.

PY - 1981/6

Y1 - 1981/6

N2 - The nutritional parameters controlling the yeast-mycelial dimorphism in Ceratocystis ulmi, the causative agent of Dutch elm disease, have been established. The nitrogen source is critical. In a defined glucose-salts liquid medium the presence of proline induces the yeast morphology whereas ammonium, arginine, or asparagine induces the mycelial state. This phenomenon is not a secondary manifestation of pH changes in the respective media and it can be achieved with either a blastospore or a conidiospore inoculum. The dimorphism is, however, dependent on the inoculum size. Yeasts are only formed in the proline-containing medium with inocula giving cell concentrations of ≥106 blastospores/ml. Once the spores produce visible buds or germ tubes they are "committed" to that developmental pattern in the sense that if they are now resuspended in the opposite medium, at the same cell concentration, the alternate morphology can no longer be produced.

AB - The nutritional parameters controlling the yeast-mycelial dimorphism in Ceratocystis ulmi, the causative agent of Dutch elm disease, have been established. The nitrogen source is critical. In a defined glucose-salts liquid medium the presence of proline induces the yeast morphology whereas ammonium, arginine, or asparagine induces the mycelial state. This phenomenon is not a secondary manifestation of pH changes in the respective media and it can be achieved with either a blastospore or a conidiospore inoculum. The dimorphism is, however, dependent on the inoculum size. Yeasts are only formed in the proline-containing medium with inocula giving cell concentrations of ≥106 blastospores/ml. Once the spores produce visible buds or germ tubes they are "committed" to that developmental pattern in the sense that if they are now resuspended in the opposite medium, at the same cell concentration, the alternate morphology can no longer be produced.

KW - Ceratocystis ulmi

KW - Dutch elm disease

KW - fungal nitrogen metabolism

KW - yeast-mycelial dimorphism

UR - http://www.scopus.com/inward/record.url?scp=0011155266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0011155266&partnerID=8YFLogxK

U2 - 10.1016/0147-5975(81)90015-3

DO - 10.1016/0147-5975(81)90015-3

M3 - Article

AN - SCOPUS:0011155266

VL - 5

SP - 148

EP - 154

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

IS - 2

ER -