Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene

David Rockabrand, Jim Partridge, Julie Krska, Paul Blum

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

DNA sequence analysis of chromosomal DNA from the Gram-positive facultative intracellular pathogen, Erysipelothrix rhusiopathiae has identified a dnaJ heat shock gene homolog. A 1109-bp open reading frame encoding dnaJ is located immediately 3′ to the E. rhusiopathiae dnaK gene. The deduced DnaJ amino acid sequence exhibits the modular structure of other members of the DnaJ protein class including a glycine-rich region and the repeating consensus sequence CXXCXGXGX. Heterologous expression of the dnaJ sequence in Escherichia coli resulted in accumulation of a unique 38.9-kDa protein with an isoelectric point of 8.0. Deletion analysis of the dnaJ gene was used to confirm that the overproduced protein was encoded by the dnaJ sequence.

Original languageEnglish (US)
Pages (from-to)79-85
Number of pages7
JournalFEMS Microbiology Letters
Volume111
Issue number1
DOIs
StatePublished - Jul 15 1993

Fingerprint

Erysipelothrix
Sequence Analysis
HSP40 Heat-Shock Proteins
Genes
Consensus Sequence
Isoelectric Point
DNA Sequence Analysis
Glycine
Open Reading Frames
Amino Acid Sequence
Shock
Proteins
Hot Temperature
Escherichia coli
DNA

Keywords

  • DnaJ
  • Erysipelothrix rhusiopathiae
  • Heat-shock
  • Pathogen

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene. / Rockabrand, David; Partridge, Jim; Krska, Julie; Blum, Paul.

In: FEMS Microbiology Letters, Vol. 111, No. 1, 15.07.1993, p. 79-85.

Research output: Contribution to journalArticle

Rockabrand, David ; Partridge, Jim ; Krska, Julie ; Blum, Paul. / Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene. In: FEMS Microbiology Letters. 1993 ; Vol. 111, No. 1. pp. 79-85.
@article{03a77741afe548eabc7cee8174d32ef2,
title = "Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene",
abstract = "DNA sequence analysis of chromosomal DNA from the Gram-positive facultative intracellular pathogen, Erysipelothrix rhusiopathiae has identified a dnaJ heat shock gene homolog. A 1109-bp open reading frame encoding dnaJ is located immediately 3′ to the E. rhusiopathiae dnaK gene. The deduced DnaJ amino acid sequence exhibits the modular structure of other members of the DnaJ protein class including a glycine-rich region and the repeating consensus sequence CXXCXGXGX. Heterologous expression of the dnaJ sequence in Escherichia coli resulted in accumulation of a unique 38.9-kDa protein with an isoelectric point of 8.0. Deletion analysis of the dnaJ gene was used to confirm that the overproduced protein was encoded by the dnaJ sequence.",
keywords = "DnaJ, Erysipelothrix rhusiopathiae, Heat-shock, Pathogen",
author = "David Rockabrand and Jim Partridge and Julie Krska and Paul Blum",
year = "1993",
month = "7",
day = "15",
doi = "10.1111/j.1574-6968.1993.tb06365.x",
language = "English (US)",
volume = "111",
pages = "79--85",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Nucleotide sequence analysis and heterologous expression of the Erysipelothrix rhusiopathiae dnaJ gene

AU - Rockabrand, David

AU - Partridge, Jim

AU - Krska, Julie

AU - Blum, Paul

PY - 1993/7/15

Y1 - 1993/7/15

N2 - DNA sequence analysis of chromosomal DNA from the Gram-positive facultative intracellular pathogen, Erysipelothrix rhusiopathiae has identified a dnaJ heat shock gene homolog. A 1109-bp open reading frame encoding dnaJ is located immediately 3′ to the E. rhusiopathiae dnaK gene. The deduced DnaJ amino acid sequence exhibits the modular structure of other members of the DnaJ protein class including a glycine-rich region and the repeating consensus sequence CXXCXGXGX. Heterologous expression of the dnaJ sequence in Escherichia coli resulted in accumulation of a unique 38.9-kDa protein with an isoelectric point of 8.0. Deletion analysis of the dnaJ gene was used to confirm that the overproduced protein was encoded by the dnaJ sequence.

AB - DNA sequence analysis of chromosomal DNA from the Gram-positive facultative intracellular pathogen, Erysipelothrix rhusiopathiae has identified a dnaJ heat shock gene homolog. A 1109-bp open reading frame encoding dnaJ is located immediately 3′ to the E. rhusiopathiae dnaK gene. The deduced DnaJ amino acid sequence exhibits the modular structure of other members of the DnaJ protein class including a glycine-rich region and the repeating consensus sequence CXXCXGXGX. Heterologous expression of the dnaJ sequence in Escherichia coli resulted in accumulation of a unique 38.9-kDa protein with an isoelectric point of 8.0. Deletion analysis of the dnaJ gene was used to confirm that the overproduced protein was encoded by the dnaJ sequence.

KW - DnaJ

KW - Erysipelothrix rhusiopathiae

KW - Heat-shock

KW - Pathogen

UR - http://www.scopus.com/inward/record.url?scp=0027220876&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027220876&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.1993.tb06365.x

DO - 10.1111/j.1574-6968.1993.tb06365.x

M3 - Article

C2 - 8359682

AN - SCOPUS:0027220876

VL - 111

SP - 79

EP - 85

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -