Nucleotide excision repair in the third kingdom

M. Ogrunc, D. F. Becker, S. W. Ragsdale, A. Sancar

Research output: Contribution to journalArticle

33 Scopus citations


Nucleotide excision repair, a general repair mechanism for removing DNA damage, is initiated by dual incisions bracketing the lesion. In procaryotes, the dual incisions result in excision of the damage in 12- to 13-nucleotide- long oligomers, and in eucaryotes they result in excision of the damage in the form of 24- to 32-nucleotide-long oligomers. We wished to find out if Archaea perform excision repair. Using cell extracts from Methanobacterium thermoautotrophicum, we found that this organism removes UV-induced (6-4) photoproducts in the form of 10- to 11-mers by incising the sixth to seventh phosphodiester bond 5' to the damage and the fourth phosphodiester bond 3' to the damage.

Original languageEnglish (US)
Pages (from-to)5796-5798
Number of pages3
JournalJournal of bacteriology
Issue number21
StatePublished - Nov 7 1998


ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Ogrunc, M., Becker, D. F., Ragsdale, S. W., & Sancar, A. (1998). Nucleotide excision repair in the third kingdom. Journal of bacteriology, 180(21), 5796-5798.