Nucleosomal organization of a part of chromatin in mollusc sperm nuclei with a mixed basic protein composition

A. O. Zalensky, Z. V. Avramova

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The structural organization of mature sperm chromatin from three representatives of the Mytilidae family has been studied. The acid-soluble proteins in these species nuclei are primarily sperm-specific (approximately 80%) with the remainder being core histones. Previously, we have shown that the mature sperm nuclei of these molluscs are compact, dense structures formed by interaction of the spermspecific proteins with DNA (1). Here we show that: a) although the histones are minor chromatin protein fraction, they still organize a part (20-25%) of the total DNA into nucleosomes; b) one of the sperm-specific proteins, different from somatic H1 or H5 histones participates in the formation of the beaded structures.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalMolecular Biology Reports
Volume10
Issue number2
DOIs
StatePublished - Dec 1 1984

Fingerprint

Molluscs
Mollusca
Histones
Chromatin
Spermatozoa
Chemical analysis
Mytilidae
Proteins
Nucleosomes
DNA
Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nucleosomal organization of a part of chromatin in mollusc sperm nuclei with a mixed basic protein composition. / Zalensky, A. O.; Avramova, Z. V.

In: Molecular Biology Reports, Vol. 10, No. 2, 01.12.1984, p. 69-74.

Research output: Contribution to journalArticle

@article{c6accda6f67e41c39eb81d4343bf3687,
title = "Nucleosomal organization of a part of chromatin in mollusc sperm nuclei with a mixed basic protein composition",
abstract = "The structural organization of mature sperm chromatin from three representatives of the Mytilidae family has been studied. The acid-soluble proteins in these species nuclei are primarily sperm-specific (approximately 80{\%}) with the remainder being core histones. Previously, we have shown that the mature sperm nuclei of these molluscs are compact, dense structures formed by interaction of the spermspecific proteins with DNA (1). Here we show that: a) although the histones are minor chromatin protein fraction, they still organize a part (20-25{\%}) of the total DNA into nucleosomes; b) one of the sperm-specific proteins, different from somatic H1 or H5 histones participates in the formation of the beaded structures.",
author = "Zalensky, {A. O.} and Avramova, {Z. V.}",
year = "1984",
month = "12",
day = "1",
doi = "10.1007/BF00776976",
language = "English (US)",
volume = "10",
pages = "69--74",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Nucleosomal organization of a part of chromatin in mollusc sperm nuclei with a mixed basic protein composition

AU - Zalensky, A. O.

AU - Avramova, Z. V.

PY - 1984/12/1

Y1 - 1984/12/1

N2 - The structural organization of mature sperm chromatin from three representatives of the Mytilidae family has been studied. The acid-soluble proteins in these species nuclei are primarily sperm-specific (approximately 80%) with the remainder being core histones. Previously, we have shown that the mature sperm nuclei of these molluscs are compact, dense structures formed by interaction of the spermspecific proteins with DNA (1). Here we show that: a) although the histones are minor chromatin protein fraction, they still organize a part (20-25%) of the total DNA into nucleosomes; b) one of the sperm-specific proteins, different from somatic H1 or H5 histones participates in the formation of the beaded structures.

AB - The structural organization of mature sperm chromatin from three representatives of the Mytilidae family has been studied. The acid-soluble proteins in these species nuclei are primarily sperm-specific (approximately 80%) with the remainder being core histones. Previously, we have shown that the mature sperm nuclei of these molluscs are compact, dense structures formed by interaction of the spermspecific proteins with DNA (1). Here we show that: a) although the histones are minor chromatin protein fraction, they still organize a part (20-25%) of the total DNA into nucleosomes; b) one of the sperm-specific proteins, different from somatic H1 or H5 histones participates in the formation of the beaded structures.

UR - http://www.scopus.com/inward/record.url?scp=0021666754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021666754&partnerID=8YFLogxK

U2 - 10.1007/BF00776976

DO - 10.1007/BF00776976

M3 - Article

C2 - 6527685

AN - SCOPUS:0021666754

VL - 10

SP - 69

EP - 74

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 2

ER -