Nucleoside transporter-mediated uptake and release of [3H]L-adenosine in DDT1 MF-2 smooth muscle cells

Irene O. Foga, Jonathan D. Geiger, Fiona E. Parkinson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

[3H]L-Adenosine, an enantiomer of the physiological D-adenosine, was shown previously to be taken up and released, at least in part, through nucleoside transporters in rat brain preparations. In the present study, we used clonal smooth muscle DDT1 MF-2 cells that contain almost exclusively equilibrative inhibitor-sensitive (es) nucleoside transporters to test the hypothesis that L-adenosine is a permeant for these bidirectional nucleoside transporters. DDT1 MF-2 cells accumulated approximately 3 times more [3H]D- than [3H]L-adenosine; 10 μM nitrobenzylthioinosine significantly (P < 0.01) inhibited the accumulation of [3H]D-adenosine by 86% and of [3H]L-adenosine by 63%. The IC50 values for the nitrobenzylthioinosine-sensitive portions of [3H]L- and [3H]D-adenosine accumulation were 1.6 and 2.0 nM, respectively. [3H]D-Adenosine accumulation was significantly (P < 0.05) inhibited by up to 72% with L-adenosine and [3H]L-adenosine accumulation was significantly (P < 0.01) inhibited by up to 52% with D-adenosine. Release of accumulated [3H]L-adenosine was temperature- and time-dependent, and was significantly (P < 0.05) reduced by 47% with dipyridamole, 39% with dilazep, and 45% with nitrobenzylthioinosine; the apparent IC50 value for nitrobenzylthioinosine was < 1 nM. These data indicate that a significant proportion of [3H]L-adenosine uptake and release in DDT1 MF-2 cells is mediated by es nucleoside transporters.

Original languageEnglish (US)
Pages (from-to)455-460
Number of pages6
JournalEuropean Journal of Pharmacology
Volume318
Issue number2-3
DOIs
StatePublished - Dec 30 1996

Fingerprint

Nucleoside Transport Proteins
Adenosine
Smooth Muscle Myocytes
Inhibitory Concentration 50
Dilazep
Dipyridamole

Keywords

  • Adenosine
  • L-Adenosine
  • Nitrobenzylthioinosine
  • Nucleoside transport

ASJC Scopus subject areas

  • Pharmacology

Cite this

Nucleoside transporter-mediated uptake and release of [3H]L-adenosine in DDT1 MF-2 smooth muscle cells. / Foga, Irene O.; Geiger, Jonathan D.; Parkinson, Fiona E.

In: European Journal of Pharmacology, Vol. 318, No. 2-3, 30.12.1996, p. 455-460.

Research output: Contribution to journalArticle

@article{d1cd3b63edec476aa9145cbaac125adb,
title = "Nucleoside transporter-mediated uptake and release of [3H]L-adenosine in DDT1 MF-2 smooth muscle cells",
abstract = "[3H]L-Adenosine, an enantiomer of the physiological D-adenosine, was shown previously to be taken up and released, at least in part, through nucleoside transporters in rat brain preparations. In the present study, we used clonal smooth muscle DDT1 MF-2 cells that contain almost exclusively equilibrative inhibitor-sensitive (es) nucleoside transporters to test the hypothesis that L-adenosine is a permeant for these bidirectional nucleoside transporters. DDT1 MF-2 cells accumulated approximately 3 times more [3H]D- than [3H]L-adenosine; 10 μM nitrobenzylthioinosine significantly (P < 0.01) inhibited the accumulation of [3H]D-adenosine by 86{\%} and of [3H]L-adenosine by 63{\%}. The IC50 values for the nitrobenzylthioinosine-sensitive portions of [3H]L- and [3H]D-adenosine accumulation were 1.6 and 2.0 nM, respectively. [3H]D-Adenosine accumulation was significantly (P < 0.05) inhibited by up to 72{\%} with L-adenosine and [3H]L-adenosine accumulation was significantly (P < 0.01) inhibited by up to 52{\%} with D-adenosine. Release of accumulated [3H]L-adenosine was temperature- and time-dependent, and was significantly (P < 0.05) reduced by 47{\%} with dipyridamole, 39{\%} with dilazep, and 45{\%} with nitrobenzylthioinosine; the apparent IC50 value for nitrobenzylthioinosine was < 1 nM. These data indicate that a significant proportion of [3H]L-adenosine uptake and release in DDT1 MF-2 cells is mediated by es nucleoside transporters.",
keywords = "Adenosine, L-Adenosine, Nitrobenzylthioinosine, Nucleoside transport",
author = "Foga, {Irene O.} and Geiger, {Jonathan D.} and Parkinson, {Fiona E.}",
year = "1996",
month = "12",
day = "30",
doi = "10.1016/S0014-2999(96)00720-0",
language = "English (US)",
volume = "318",
pages = "455--460",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Nucleoside transporter-mediated uptake and release of [3H]L-adenosine in DDT1 MF-2 smooth muscle cells

AU - Foga, Irene O.

AU - Geiger, Jonathan D.

AU - Parkinson, Fiona E.

PY - 1996/12/30

Y1 - 1996/12/30

N2 - [3H]L-Adenosine, an enantiomer of the physiological D-adenosine, was shown previously to be taken up and released, at least in part, through nucleoside transporters in rat brain preparations. In the present study, we used clonal smooth muscle DDT1 MF-2 cells that contain almost exclusively equilibrative inhibitor-sensitive (es) nucleoside transporters to test the hypothesis that L-adenosine is a permeant for these bidirectional nucleoside transporters. DDT1 MF-2 cells accumulated approximately 3 times more [3H]D- than [3H]L-adenosine; 10 μM nitrobenzylthioinosine significantly (P < 0.01) inhibited the accumulation of [3H]D-adenosine by 86% and of [3H]L-adenosine by 63%. The IC50 values for the nitrobenzylthioinosine-sensitive portions of [3H]L- and [3H]D-adenosine accumulation were 1.6 and 2.0 nM, respectively. [3H]D-Adenosine accumulation was significantly (P < 0.05) inhibited by up to 72% with L-adenosine and [3H]L-adenosine accumulation was significantly (P < 0.01) inhibited by up to 52% with D-adenosine. Release of accumulated [3H]L-adenosine was temperature- and time-dependent, and was significantly (P < 0.05) reduced by 47% with dipyridamole, 39% with dilazep, and 45% with nitrobenzylthioinosine; the apparent IC50 value for nitrobenzylthioinosine was < 1 nM. These data indicate that a significant proportion of [3H]L-adenosine uptake and release in DDT1 MF-2 cells is mediated by es nucleoside transporters.

AB - [3H]L-Adenosine, an enantiomer of the physiological D-adenosine, was shown previously to be taken up and released, at least in part, through nucleoside transporters in rat brain preparations. In the present study, we used clonal smooth muscle DDT1 MF-2 cells that contain almost exclusively equilibrative inhibitor-sensitive (es) nucleoside transporters to test the hypothesis that L-adenosine is a permeant for these bidirectional nucleoside transporters. DDT1 MF-2 cells accumulated approximately 3 times more [3H]D- than [3H]L-adenosine; 10 μM nitrobenzylthioinosine significantly (P < 0.01) inhibited the accumulation of [3H]D-adenosine by 86% and of [3H]L-adenosine by 63%. The IC50 values for the nitrobenzylthioinosine-sensitive portions of [3H]L- and [3H]D-adenosine accumulation were 1.6 and 2.0 nM, respectively. [3H]D-Adenosine accumulation was significantly (P < 0.05) inhibited by up to 72% with L-adenosine and [3H]L-adenosine accumulation was significantly (P < 0.01) inhibited by up to 52% with D-adenosine. Release of accumulated [3H]L-adenosine was temperature- and time-dependent, and was significantly (P < 0.05) reduced by 47% with dipyridamole, 39% with dilazep, and 45% with nitrobenzylthioinosine; the apparent IC50 value for nitrobenzylthioinosine was < 1 nM. These data indicate that a significant proportion of [3H]L-adenosine uptake and release in DDT1 MF-2 cells is mediated by es nucleoside transporters.

KW - Adenosine

KW - L-Adenosine

KW - Nitrobenzylthioinosine

KW - Nucleoside transport

UR - http://www.scopus.com/inward/record.url?scp=0030607708&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030607708&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(96)00720-0

DO - 10.1016/S0014-2999(96)00720-0

M3 - Article

C2 - 9016938

AN - SCOPUS:0030607708

VL - 318

SP - 455

EP - 460

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2-3

ER -