Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95

Kelly M. Trujillo, Shyng Shiou F. Yuan, Eva Y.H.P. Lee, Patrick Sung

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Abstract

Genetic studies in yeast have indicated a role of the RAD50 and MRE11 genes in homologous recombination, telomere length maintenance, and DNA repair processes. Here, we purify from nuclear extract of Raji cells a complex consisting of human Rad50, Mre11, and another protein factor with a size of about 95 kDa (p95), which is likely to be Nibrin, the protein encoded by the gene mutated in Nijmegen breakage syndrome. We show that the Rad50- Mre11-p95 complex possesses manganese-dependent single-stranded DNA endonuclease and 3' to 5' exonuclease activities. These nuclease activities are likely to be important for recombination, repair, and genomic stability.

Original languageEnglish (US)
Pages (from-to)21447-21450
Number of pages4
JournalJournal of Biological Chemistry
Volume273
Issue number34
DOIs
Publication statusPublished - Aug 21 1998

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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