Nuclear Matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodeling

Kadreppa Sreenath, Lakshminarasimhan Pavithra, Sandeep Singh, Surajit Sinha, Prasanta K. Dash, Siddappa Nagadenahalli Byrareddy, Udaykumar Ranga, Debashis Mitra, Samit Chattopadhyay

Research output: Contribution to journalArticle

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Abstract

Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear. Here, we show that > 98% of HIV sequences contain consensus MAR element in their promoter. We show that SMAR1 binds to the LTR MAR and reinforces transcriptional silencing by tethering the LTR MAR to nuclear matrix. SMAR1 associated HDAC1-mSin3 corepressor complex is dislodged from the LTR upon cellular activation by PMA/TNFα leading to an increase in the acetylation and a reduction in the trimethylation of histones, associated with the recruitment of RNA Polymerase II on the LTR. Overexpression of SMAR1 lead to reduction in LTR mediated transcription, both in a Tat dependent and independent manner, resulting in a decreased virion production. These results demonstrate the role of SMAR1 in regulating viral transcription by alternative compartmentalization of LTR between the nuclear matrix and chromatin.

Original languageEnglish (US)
Pages (from-to)76-85
Number of pages10
JournalVirology
Volume400
Issue number1
DOIs
StatePublished - Apr 25 2010

Fingerprint

Nuclear Matrix-Associated Proteins
HIV Long Terminal Repeat
Nuclear Matrix
Chromatin Assembly and Disassembly
Matrix Attachment Regions
Co-Repressor Proteins
Viral Genes
RNA Polymerase II
Consensus Sequence
Acetylation
Virion
Histones
Chromatin
HIV-1
HIV

Keywords

  • HIV-1
  • LTR
  • PMA
  • SMAR1
  • TNF-α
  • Transcription

ASJC Scopus subject areas

  • Virology

Cite this

Nuclear Matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodeling. / Sreenath, Kadreppa; Pavithra, Lakshminarasimhan; Singh, Sandeep; Sinha, Surajit; Dash, Prasanta K.; Byrareddy, Siddappa Nagadenahalli; Ranga, Udaykumar; Mitra, Debashis; Chattopadhyay, Samit.

In: Virology, Vol. 400, No. 1, 25.04.2010, p. 76-85.

Research output: Contribution to journalArticle

Sreenath, K, Pavithra, L, Singh, S, Sinha, S, Dash, PK, Byrareddy, SN, Ranga, U, Mitra, D & Chattopadhyay, S 2010, 'Nuclear Matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodeling', Virology, vol. 400, no. 1, pp. 76-85. https://doi.org/10.1016/j.virol.2010.01.017
Sreenath, Kadreppa ; Pavithra, Lakshminarasimhan ; Singh, Sandeep ; Sinha, Surajit ; Dash, Prasanta K. ; Byrareddy, Siddappa Nagadenahalli ; Ranga, Udaykumar ; Mitra, Debashis ; Chattopadhyay, Samit. / Nuclear Matrix protein SMAR1 represses HIV-1 LTR mediated transcription through chromatin remodeling. In: Virology. 2010 ; Vol. 400, No. 1. pp. 76-85.
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