Nuclear expression of an environmentally friendly synthetic protein based polymer gene in tobacco cells

X. Zhang, C. Guda, R. Datta, R. Dute, D. W. Urry, H. Daniell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report here expression of a protein based polymer gene (Gly-Val-Gly-Val-Pro)121, coding for three amino acids in a pentamer sequence repeated 121 times via the nuclear genome of tobacco cells. Transformed tobacco cells were obtained by particle bombardment. Stably transformed cells show the presence of the polymer gene in the tobacco nuclear genome (2-5 copies); introduced polymer gene is transcribed efficiently as revealed by Northern blots; Western blots show the presence of the polymer protein. To the best of our knowledge, this report represents the first demonstration of expression of a synthetic gene (with no natural analog) in higher plants.

Original languageEnglish (US)
Pages (from-to)1279-1284
Number of pages6
JournalBiotechnology Letters
Volume17
Issue number12
DOIs
StatePublished - Dec 1 1995

Fingerprint

Tobacco
Polymers
Genes
Proteins
glycyl-valyl-glycyl-valyl-proline
Genome
Synthetic Genes
Northern Blotting
Western Blotting
Amino Acids
Amino acids
Demonstrations

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Nuclear expression of an environmentally friendly synthetic protein based polymer gene in tobacco cells. / Zhang, X.; Guda, C.; Datta, R.; Dute, R.; Urry, D. W.; Daniell, H.

In: Biotechnology Letters, Vol. 17, No. 12, 01.12.1995, p. 1279-1284.

Research output: Contribution to journalArticle

Zhang, X. ; Guda, C. ; Datta, R. ; Dute, R. ; Urry, D. W. ; Daniell, H. / Nuclear expression of an environmentally friendly synthetic protein based polymer gene in tobacco cells. In: Biotechnology Letters. 1995 ; Vol. 17, No. 12. pp. 1279-1284.
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