Novel wheat protein films as substrates for tissue engineering

Narendra Reddy, Qiuran Jiang, Yiqi Yang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This paper demonstrates that gliadin-free wheat glutenin can be an excellent biomaterial for tissue-engineering applications, better than poly(lactic acid) (PLA). Although plant proteins are more readily available than collagen and silk, limited studies have been conducted on understanding the potential of using plant proteins as biomaterials. Wheat proteins have not been used for tissue engineering due to the cytotoxicity of gliadin. In this research, wheat gluten, glutenin and gliadin were used to develop films and the mechanical properties, water stability and ability of the films to promote the attachment, growth and viability of osteoblast cells was studied in comparison to PLA films. The wheat protein films have good strength ranging from 8 to 30 MPa. Gliadin films experience about 50% weight loss whereas glutenin films have about 90% weight loss after being in water (pH 7.2) for 15 days at 37°C. Gliadin is cytotoxic and the presence of gliadin restricts the cell proliferation on wheat gluten films. However, gliadin-free glutenin films show a higher rate of proliferation of osteoblast cells than the PLA films. Wheat gluten promises to be a potential substrate for tissue engineering and other medical applications.

Original languageEnglish (US)
Pages (from-to)2063-2077
Number of pages15
JournalJournal of Biomaterials Science, Polymer Edition
Volume22
Issue number15
DOIs
StatePublished - 2011

Fingerprint

Gliadin
Tissue Engineering
Tissue engineering
Triticum
Proteins
Substrates
Glutens
Plant Proteins
Biocompatible Materials
Osteoblasts
Weight Loss
Biomaterials
Cell Proliferation
Silk
Water
Motion Pictures
Bioelectric potentials
Cell proliferation
Cell Survival
Medical applications

Keywords

  • Biomaterials
  • gliadin
  • proteins
  • tissue engineering
  • wheat glutenin

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Novel wheat protein films as substrates for tissue engineering. / Reddy, Narendra; Jiang, Qiuran; Yang, Yiqi.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 22, No. 15, 2011, p. 2063-2077.

Research output: Contribution to journalArticle

@article{14badfbd0d9947fb96e36df1272104ac,
title = "Novel wheat protein films as substrates for tissue engineering",
abstract = "This paper demonstrates that gliadin-free wheat glutenin can be an excellent biomaterial for tissue-engineering applications, better than poly(lactic acid) (PLA). Although plant proteins are more readily available than collagen and silk, limited studies have been conducted on understanding the potential of using plant proteins as biomaterials. Wheat proteins have not been used for tissue engineering due to the cytotoxicity of gliadin. In this research, wheat gluten, glutenin and gliadin were used to develop films and the mechanical properties, water stability and ability of the films to promote the attachment, growth and viability of osteoblast cells was studied in comparison to PLA films. The wheat protein films have good strength ranging from 8 to 30 MPa. Gliadin films experience about 50{\%} weight loss whereas glutenin films have about 90{\%} weight loss after being in water (pH 7.2) for 15 days at 37°C. Gliadin is cytotoxic and the presence of gliadin restricts the cell proliferation on wheat gluten films. However, gliadin-free glutenin films show a higher rate of proliferation of osteoblast cells than the PLA films. Wheat gluten promises to be a potential substrate for tissue engineering and other medical applications.",
keywords = "Biomaterials, gliadin, proteins, tissue engineering, wheat glutenin",
author = "Narendra Reddy and Qiuran Jiang and Yiqi Yang",
year = "2011",
doi = "10.1163/092050610X532638",
language = "English (US)",
volume = "22",
pages = "2063--2077",
journal = "Journal of Biomaterials Science, Polymer Edition",
issn = "0920-5063",
publisher = "Taylor and Francis Ltd.",
number = "15",

}

TY - JOUR

T1 - Novel wheat protein films as substrates for tissue engineering

AU - Reddy, Narendra

AU - Jiang, Qiuran

AU - Yang, Yiqi

PY - 2011

Y1 - 2011

N2 - This paper demonstrates that gliadin-free wheat glutenin can be an excellent biomaterial for tissue-engineering applications, better than poly(lactic acid) (PLA). Although plant proteins are more readily available than collagen and silk, limited studies have been conducted on understanding the potential of using plant proteins as biomaterials. Wheat proteins have not been used for tissue engineering due to the cytotoxicity of gliadin. In this research, wheat gluten, glutenin and gliadin were used to develop films and the mechanical properties, water stability and ability of the films to promote the attachment, growth and viability of osteoblast cells was studied in comparison to PLA films. The wheat protein films have good strength ranging from 8 to 30 MPa. Gliadin films experience about 50% weight loss whereas glutenin films have about 90% weight loss after being in water (pH 7.2) for 15 days at 37°C. Gliadin is cytotoxic and the presence of gliadin restricts the cell proliferation on wheat gluten films. However, gliadin-free glutenin films show a higher rate of proliferation of osteoblast cells than the PLA films. Wheat gluten promises to be a potential substrate for tissue engineering and other medical applications.

AB - This paper demonstrates that gliadin-free wheat glutenin can be an excellent biomaterial for tissue-engineering applications, better than poly(lactic acid) (PLA). Although plant proteins are more readily available than collagen and silk, limited studies have been conducted on understanding the potential of using plant proteins as biomaterials. Wheat proteins have not been used for tissue engineering due to the cytotoxicity of gliadin. In this research, wheat gluten, glutenin and gliadin were used to develop films and the mechanical properties, water stability and ability of the films to promote the attachment, growth and viability of osteoblast cells was studied in comparison to PLA films. The wheat protein films have good strength ranging from 8 to 30 MPa. Gliadin films experience about 50% weight loss whereas glutenin films have about 90% weight loss after being in water (pH 7.2) for 15 days at 37°C. Gliadin is cytotoxic and the presence of gliadin restricts the cell proliferation on wheat gluten films. However, gliadin-free glutenin films show a higher rate of proliferation of osteoblast cells than the PLA films. Wheat gluten promises to be a potential substrate for tissue engineering and other medical applications.

KW - Biomaterials

KW - gliadin

KW - proteins

KW - tissue engineering

KW - wheat glutenin

UR - http://www.scopus.com/inward/record.url?scp=80052067993&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052067993&partnerID=8YFLogxK

U2 - 10.1163/092050610X532638

DO - 10.1163/092050610X532638

M3 - Article

C2 - 21029519

AN - SCOPUS:80052067993

VL - 22

SP - 2063

EP - 2077

JO - Journal of Biomaterials Science, Polymer Edition

JF - Journal of Biomaterials Science, Polymer Edition

SN - 0920-5063

IS - 15

ER -