Novel system to study dermal-hepatic interactions in vitro

R. M. Brand, T. L. Hannah, Frederick G Hamel

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An apparatus containing both skin diffusion and cell culture chambers has been developed in our laboratory. A piece of skin is placed in a flow-through diffusion chamber with the epidermis exposed to a test compound. The compound penetrates through the skin and partitions from the dermis into a flowing buffer. The moving stream then proceeds to a cell chamber containing HEP-G2 (liver) cells. Effluent from the cell chamber is collected and assayed for glucose oxidation. This system enables the direct examination of dermal-hepatic relationships without the complexities of an in vivo system. It can also be used with other diffusional barriers or cell types.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Skin
Cell culture
Liver
Glucose
Effluents
Buffers
Oxidation

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

Brand, R. M., Hannah, T. L., & Hamel, F. G. (1999). Novel system to study dermal-hepatic interactions in vitro. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1). IEEE.

Novel system to study dermal-hepatic interactions in vitro. / Brand, R. M.; Hannah, T. L.; Hamel, Frederick G.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Brand, RM, Hannah, TL & Hamel, FG 1999, Novel system to study dermal-hepatic interactions in vitro. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 1, IEEE, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Brand RM, Hannah TL, Hamel FG. Novel system to study dermal-hepatic interactions in vitro. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE. 1999. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).
Brand, R. M. ; Hannah, T. L. ; Hamel, Frederick G. / Novel system to study dermal-hepatic interactions in vitro. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).
@inproceedings{73e26f5b15bd482aace4e0f9a31f96cd,
title = "Novel system to study dermal-hepatic interactions in vitro",
abstract = "An apparatus containing both skin diffusion and cell culture chambers has been developed in our laboratory. A piece of skin is placed in a flow-through diffusion chamber with the epidermis exposed to a test compound. The compound penetrates through the skin and partitions from the dermis into a flowing buffer. The moving stream then proceeds to a cell chamber containing HEP-G2 (liver) cells. Effluent from the cell chamber is collected and assayed for glucose oxidation. This system enables the direct examination of dermal-hepatic relationships without the complexities of an in vivo system. It can also be used with other diffusional barriers or cell types.",
author = "Brand, {R. M.} and Hannah, {T. L.} and Hamel, {Frederick G}",
year = "1999",
month = "12",
day = "1",
language = "English (US)",
isbn = "0780356756",
series = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",
publisher = "IEEE",
booktitle = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

}

TY - GEN

T1 - Novel system to study dermal-hepatic interactions in vitro

AU - Brand, R. M.

AU - Hannah, T. L.

AU - Hamel, Frederick G

PY - 1999/12/1

Y1 - 1999/12/1

N2 - An apparatus containing both skin diffusion and cell culture chambers has been developed in our laboratory. A piece of skin is placed in a flow-through diffusion chamber with the epidermis exposed to a test compound. The compound penetrates through the skin and partitions from the dermis into a flowing buffer. The moving stream then proceeds to a cell chamber containing HEP-G2 (liver) cells. Effluent from the cell chamber is collected and assayed for glucose oxidation. This system enables the direct examination of dermal-hepatic relationships without the complexities of an in vivo system. It can also be used with other diffusional barriers or cell types.

AB - An apparatus containing both skin diffusion and cell culture chambers has been developed in our laboratory. A piece of skin is placed in a flow-through diffusion chamber with the epidermis exposed to a test compound. The compound penetrates through the skin and partitions from the dermis into a flowing buffer. The moving stream then proceeds to a cell chamber containing HEP-G2 (liver) cells. Effluent from the cell chamber is collected and assayed for glucose oxidation. This system enables the direct examination of dermal-hepatic relationships without the complexities of an in vivo system. It can also be used with other diffusional barriers or cell types.

UR - http://www.scopus.com/inward/record.url?scp=0033322777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033322777&partnerID=8YFLogxK

M3 - Conference contribution

SN - 0780356756

T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

PB - IEEE

ER -