Novel assay for determining the metabolic fate of juvenile hormone III: A study with Drosophila melanogaster

J. A. Ottea, L. G. Harshman, Bruce D. Hammock

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A thin‐layer chromatographic assay was developed for the resolution of hydrolytic and conjugative catabolites of juvenile hormone (JH). A single‐dimension, dual‐development thin‐layer system allowed complete resolution of the catabolites. Thus, this system provided a means for the rapid and economic analysis of JH hydrolysis even when different hydrolytic activities were present concurrently. Purified hydrolytic enzymes were found to be superior to chemical methods for the generation of small amounts of standards of JH catabolites. The relative levels of activities of an epoxide hydrolase and an esterase toward JH III were found to be similar in microsomal preparations from three lines of adult Drosophila melanogaster isolated from a field population. However, selection of flies by exposure to cut orange resulted in the elevation of levels of epoxide hydrolase activities, whereas esterase levels were not affected to the same extent. The formation of the JH acid‐diol was not detected under the conditions of this study, suggesting that the JH acid and diol were not good substrates for epoxide hydrolase and juvenile hormone esterase, respectively.

Original languageEnglish (US)
Pages (from-to)25-37
Number of pages13
JournalArchives of Insect Biochemistry and Physiology
Volume8
Issue number1
DOIs
StatePublished - 1988

Fingerprint

Juvenile Hormones
juvenile hormones
Drosophila melanogaster
Assays
Epoxide Hydrolases
epoxide hydrolase
assays
Esterases
metabolites
esterases
Hydrolases
juvenile hormone esterase
Economic analysis
glycols
Diptera
Hydrolysis
economic analysis
Economics
juvenile hormone III
hydrolysis

Keywords

  • JH esterase
  • epoxide hydrolase
  • juvenile hormone

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Insect Science

Cite this

Novel assay for determining the metabolic fate of juvenile hormone III : A study with Drosophila melanogaster. / Ottea, J. A.; Harshman, L. G.; Hammock, Bruce D.

In: Archives of Insect Biochemistry and Physiology, Vol. 8, No. 1, 1988, p. 25-37.

Research output: Contribution to journalArticle

@article{af748b1c841c44ffa57de07535caecaa,
title = "Novel assay for determining the metabolic fate of juvenile hormone III: A study with Drosophila melanogaster",
abstract = "A thin‐layer chromatographic assay was developed for the resolution of hydrolytic and conjugative catabolites of juvenile hormone (JH). A single‐dimension, dual‐development thin‐layer system allowed complete resolution of the catabolites. Thus, this system provided a means for the rapid and economic analysis of JH hydrolysis even when different hydrolytic activities were present concurrently. Purified hydrolytic enzymes were found to be superior to chemical methods for the generation of small amounts of standards of JH catabolites. The relative levels of activities of an epoxide hydrolase and an esterase toward JH III were found to be similar in microsomal preparations from three lines of adult Drosophila melanogaster isolated from a field population. However, selection of flies by exposure to cut orange resulted in the elevation of levels of epoxide hydrolase activities, whereas esterase levels were not affected to the same extent. The formation of the JH acid‐diol was not detected under the conditions of this study, suggesting that the JH acid and diol were not good substrates for epoxide hydrolase and juvenile hormone esterase, respectively.",
keywords = "JH esterase, epoxide hydrolase, juvenile hormone",
author = "Ottea, {J. A.} and Harshman, {L. G.} and Hammock, {Bruce D.}",
year = "1988",
doi = "10.1002/arch.940080104",
language = "English (US)",
volume = "8",
pages = "25--37",
journal = "Archives of Insect Biochemistry and Physiology",
issn = "0739-4462",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Novel assay for determining the metabolic fate of juvenile hormone III

T2 - A study with Drosophila melanogaster

AU - Ottea, J. A.

AU - Harshman, L. G.

AU - Hammock, Bruce D.

PY - 1988

Y1 - 1988

N2 - A thin‐layer chromatographic assay was developed for the resolution of hydrolytic and conjugative catabolites of juvenile hormone (JH). A single‐dimension, dual‐development thin‐layer system allowed complete resolution of the catabolites. Thus, this system provided a means for the rapid and economic analysis of JH hydrolysis even when different hydrolytic activities were present concurrently. Purified hydrolytic enzymes were found to be superior to chemical methods for the generation of small amounts of standards of JH catabolites. The relative levels of activities of an epoxide hydrolase and an esterase toward JH III were found to be similar in microsomal preparations from three lines of adult Drosophila melanogaster isolated from a field population. However, selection of flies by exposure to cut orange resulted in the elevation of levels of epoxide hydrolase activities, whereas esterase levels were not affected to the same extent. The formation of the JH acid‐diol was not detected under the conditions of this study, suggesting that the JH acid and diol were not good substrates for epoxide hydrolase and juvenile hormone esterase, respectively.

AB - A thin‐layer chromatographic assay was developed for the resolution of hydrolytic and conjugative catabolites of juvenile hormone (JH). A single‐dimension, dual‐development thin‐layer system allowed complete resolution of the catabolites. Thus, this system provided a means for the rapid and economic analysis of JH hydrolysis even when different hydrolytic activities were present concurrently. Purified hydrolytic enzymes were found to be superior to chemical methods for the generation of small amounts of standards of JH catabolites. The relative levels of activities of an epoxide hydrolase and an esterase toward JH III were found to be similar in microsomal preparations from three lines of adult Drosophila melanogaster isolated from a field population. However, selection of flies by exposure to cut orange resulted in the elevation of levels of epoxide hydrolase activities, whereas esterase levels were not affected to the same extent. The formation of the JH acid‐diol was not detected under the conditions of this study, suggesting that the JH acid and diol were not good substrates for epoxide hydrolase and juvenile hormone esterase, respectively.

KW - JH esterase

KW - epoxide hydrolase

KW - juvenile hormone

UR - http://www.scopus.com/inward/record.url?scp=84990453220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990453220&partnerID=8YFLogxK

U2 - 10.1002/arch.940080104

DO - 10.1002/arch.940080104

M3 - Article

AN - SCOPUS:84990453220

VL - 8

SP - 25

EP - 37

JO - Archives of Insect Biochemistry and Physiology

JF - Archives of Insect Biochemistry and Physiology

SN - 0739-4462

IS - 1

ER -