Nonuniform size distribution of nascent peptides

The role of messenger RNA

William G Chaney, Allan J. Morris

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The origin of the nonuniform size distribution of nascent rabbit globin peptides has been investigated in the reticulocyte lysate and wheat germ cell-free protein synthesis systems. Increasing the concentrations of the cellular components involved in protein synthesis failed to alter the elution pattern observed upon chromatographic analysis of reticulocyte lysate nascent chains. Nascent chains isolated from a globin messenger RNA-directed wheat germ cell-free system showed a nonuniform size distribution of nascent peptides similar to that of the rabbit reticulocyte nascent chains. These observations indicate that the nonuniformity of the globin nascent chains arises from a unique property of the messenger RNA being translated and not from limiting concentrations of a component or components of the reticulocyte protein synthesis system.

Original languageEnglish (US)
Pages (from-to)734-741
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume191
Issue number2
DOIs
StatePublished - Jan 1 1978

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Globins
Reticulocytes
Messenger RNA
Peptides
Cells
Germ Cells
Chromatographic analysis
Triticum
Proteins
Rabbits
Cell-Free System
Chromatography

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Nonuniform size distribution of nascent peptides : The role of messenger RNA. / Chaney, William G; Morris, Allan J.

In: Archives of Biochemistry and Biophysics, Vol. 191, No. 2, 01.01.1978, p. 734-741.

Research output: Contribution to journalArticle

Chaney, William G ; Morris, Allan J. / Nonuniform size distribution of nascent peptides : The role of messenger RNA. In: Archives of Biochemistry and Biophysics. 1978 ; Vol. 191, No. 2. pp. 734-741.
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