Nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types

E. J.H. Ross, L. Shearman, M. Mathiesen, Y. J. Zhou, R. Arredondo-Peter, G. Sarath, R. V. Klucas

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Nonsymbiotic hemoglobins (ns-Hbs) previously have been found in monocots and dicots; however, very little is known about the tissue and cell type localization as well as the physiological function(s) of these oxygen-binding proteins. We report the immunodetection and immunolocalization of ns-Hbs in rice (Oryza sativa L.) by Western blotting and in situ confocal laser scanning techniques. Ns-Hbs were detected in soluble extracts of different tissues from the developing rice seedling by immunoblotting. Levels of ns-Hbs increased in the germinating seed for the first six days following imbibition and remained relatively constant thereafter. In contrast, ns-Hb levels decreased during leaf maturation. Roots and mesocotyls contained detectable, but low levels of ns-Hbs. Split-seed experiments revealed that ns-Hbs are synthesized de novo during seed germination and are expressed in the absence of any signal originating from the embryo. Immunolocalization of ns-Hbs by confocal microscopy indicated the presence of ns-Hbs primarily in differentiated and differentiating cell types of the developing seedling, such as the aleurone, scutellum, root cap cells, sclerenchyma, and tracheary elements. To our knowledge, this is the first report of the specific cellular localization of these proteins during seedling development.

Original languageEnglish (US)
Pages (from-to)125-133
Number of pages9
JournalProtoplasma
Volume218
Issue number3-4
DOIs
StatePublished - Dec 18 2001

Fingerprint

Germination
hemoglobin
Hemoglobins
germination
rice
Seedlings
Seeds
cells
seedlings
mesocotyls
tracheary elements
sclerenchyma
root cap
imbibition
Tissue Extracts
Magnoliopsida
Liliopsida
immunoblotting
seeds
embryo (plant)

Keywords

  • Confocal microscopy
  • Immunodetection
  • Nonsymbiotic hemoglobin
  • Oryza sativa L.
  • Plant development
  • Seed germination

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Ross, E. J. H., Shearman, L., Mathiesen, M., Zhou, Y. J., Arredondo-Peter, R., Sarath, G., & Klucas, R. V. (2001). Nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types. Protoplasma, 218(3-4), 125-133. https://doi.org/10.1007/BF01306602

Nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types. / Ross, E. J.H.; Shearman, L.; Mathiesen, M.; Zhou, Y. J.; Arredondo-Peter, R.; Sarath, G.; Klucas, R. V.

In: Protoplasma, Vol. 218, No. 3-4, 18.12.2001, p. 125-133.

Research output: Contribution to journalArticle

Ross, EJH, Shearman, L, Mathiesen, M, Zhou, YJ, Arredondo-Peter, R, Sarath, G & Klucas, RV 2001, 'Nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types', Protoplasma, vol. 218, no. 3-4, pp. 125-133. https://doi.org/10.1007/BF01306602
Ross EJH, Shearman L, Mathiesen M, Zhou YJ, Arredondo-Peter R, Sarath G et al. Nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types. Protoplasma. 2001 Dec 18;218(3-4):125-133. https://doi.org/10.1007/BF01306602
Ross, E. J.H. ; Shearman, L. ; Mathiesen, M. ; Zhou, Y. J. ; Arredondo-Peter, R. ; Sarath, G. ; Klucas, R. V. / Nonsymbiotic hemoglobins in rice are synthesized during germination and in differentiating cell types. In: Protoplasma. 2001 ; Vol. 218, No. 3-4. pp. 125-133.
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