Nonenzymatic glycosylation of lepidopteran-active Bacillus thuringiensis protein crystals

M. Bhattacharya, B. A. Plantz, J. D. Swanson-Kobler, Kenneth Nickerson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We used high-pH anion-exchange chromatography with pulsed amperometric detection to quantify the monosaccharides covalently attached to Bacillus thuringiensis HD-1 (Dipel) crystals. The crystals contained 0.54% sugars, including, in decreasing order of prevalence, glucose, fucose, arabinose/rhamnose, galactose, galactosamine, glucosamine, xylose, and mannose. Three lines of evidence indicated that these sugars arose from nonenzymatic glycosylation: (i) the sugars could not be removed by N- or O- glycanases; (ii) the sugars attached were influenced both by the medium in which the bacteria had been grown and by the time at which the crystals were harvested; and (iii) the chemical identity and stoichiometry of the sugars detected did not fit any known glycoprotein models. Thus, the sugars detected were the product of fermentation conditions rather than bacterial genetics. The implications of these findings are discussed in terms of crystal chemistry, fermentation technology, and the efficacy of B. thuringiensis as a microbial insecticide.

Original languageEnglish (US)
Pages (from-to)2666-2672
Number of pages7
JournalApplied and environmental microbiology
Volume59
Issue number8
StatePublished - Jan 1 1993

Fingerprint

crystal proteins
Bacillus thuringiensis
glycosylation
Glycosylation
sugar
Lepidoptera
crystal
sugars
Fermentation
protein
crystals
Rhamnose
Galactosamine
Arabinose
Fucose
Monosaccharides
Xylose
Glucosamine
Mannose
Insecticides

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Nonenzymatic glycosylation of lepidopteran-active Bacillus thuringiensis protein crystals. / Bhattacharya, M.; Plantz, B. A.; Swanson-Kobler, J. D.; Nickerson, Kenneth.

In: Applied and environmental microbiology, Vol. 59, No. 8, 01.01.1993, p. 2666-2672.

Research output: Contribution to journalArticle

Bhattacharya, M. ; Plantz, B. A. ; Swanson-Kobler, J. D. ; Nickerson, Kenneth. / Nonenzymatic glycosylation of lepidopteran-active Bacillus thuringiensis protein crystals. In: Applied and environmental microbiology. 1993 ; Vol. 59, No. 8. pp. 2666-2672.
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