Nondestructive estimation of anthocyanins and chlorophylls in anthocyanic leaves

Anatoly A. Gitelson, Olga B. Chivkunova, Mark N. Merzlyak

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The anthocyanin and chlorophyll contents in leaves provide valuable information about the physiological status of plants. Thus, there is a need for accurate, efficient, and practical methodologies to estimate these biochemical parameters of vegetation. In this study, we tested the performance and accuracy of several nondestructive, reflectance-based techniques for estimating antho-cyanin and chlorophyll contents in leaves of four unrelated species, European hazel (Corylus avellana), Siberian dogwood (Cornus alba =Swida alba), Norway maple (Acer platanoides), and Virginia creeper (Parthenocissus quinquefolia), with widely variable pigment content and composition. An anthocyanin reflectance index, which uses reflectances in the green and red edge spectral bands, and a modified anthocyanin reflectance index, employing, in addition, the near-infrared (NIR) band, were able to accurately estimate leaf anthocyanin for all species taken together with no reparameterization of algorithms. Total chlorophyll content was accurately estimated by a red edge chlorophyll index that uses spectral bands in the red edge and the NIR. These approaches can be used to estimate anthocyanin and chlorophyll nondestructively and allow the development of simple handheld field instrumentation.

Original languageEnglish (US)
Pages (from-to)1861-1868
Number of pages8
JournalAmerican Journal of Botany
Volume96
Issue number10
DOIs
StatePublished - Oct 1 2009

Fingerprint

Anthocyanins
Chlorophyll
anthocyanins
chlorophyll
reflectance
Cornus alba
Parthenocissus quinquefolia
Cornus
Acer
Acer platanoides
leaves
near infrared
cyanin
Corylus
Corylus avellana
instrumentation
Norway
physiological state
pigment
pigments

Keywords

  • Anthocyanins
  • Chlorophyll
  • Leaves
  • Nondestructive estimation
  • Reflectance

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science

Cite this

Nondestructive estimation of anthocyanins and chlorophylls in anthocyanic leaves. / Gitelson, Anatoly A.; Chivkunova, Olga B.; Merzlyak, Mark N.

In: American Journal of Botany, Vol. 96, No. 10, 01.10.2009, p. 1861-1868.

Research output: Contribution to journalArticle

Gitelson, Anatoly A. ; Chivkunova, Olga B. ; Merzlyak, Mark N. / Nondestructive estimation of anthocyanins and chlorophylls in anthocyanic leaves. In: American Journal of Botany. 2009 ; Vol. 96, No. 10. pp. 1861-1868.
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