Non-toxic and clean crosslinking system for protein materials: Effect of extenders on crosslinking performance

Kaili Song, Helan Xu, Bingnan Mu, Kongliang Xie, Yiqi Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

One of the biggest disadvantages of protein cross-linking method is the toxicity of cross-linking agents. In this paper, a clean and non-toxic cross-linking system for protein materials using citric acid (CA) as crosslinker and polyols as cross-linking extender was established. Incorporation of polyols could enhance cross-linking performance of CA by forming branched network among protein molecules. Effect of polyol structure on the cross-linking performance was studied systematically, and keratin fiber was used as an example of protein materials. Extenders containing both primary and secondary hydroxyl groups were more effective in enhancing cross-linking efficiency. Changes in the amounts of carboxyl group, amine group and sulfhydryl group of keratin fiber after cross-linking were measured via potentiometric and conductometric titrations to quantify the cross-linking efficiency. Cross-linking mechanism was also illustrated. With proper numbers of primary hydroxyl groups, secondary hydroxyl groups and backbone length, polyols could be efficient in enhancing performance of this cross-linking system. This novel clean cross-linking system could be used for not only protein engineering, but also other areas where utilization of cross-linking agents are contemplated.

Original languageEnglish (US)
Pages (from-to)214-223
Number of pages10
JournalJournal of Cleaner Production
Volume150
DOIs
StatePublished - May 1 2017

Fingerprint

Crosslinking
Polyols
Proteins
protein
Keratin
citric acid
Citric acid
Fibers
Titration
Toxicity
Amines
effect
material
Protein
toxicity
engineering
Molecules
fibre

Keywords

  • Citric acid
  • Keratin fiber
  • Non-toxic cross-linking
  • Polyol

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)
  • Strategy and Management
  • Industrial and Manufacturing Engineering

Cite this

Non-toxic and clean crosslinking system for protein materials : Effect of extenders on crosslinking performance. / Song, Kaili; Xu, Helan; Mu, Bingnan; Xie, Kongliang; Yang, Yiqi.

In: Journal of Cleaner Production, Vol. 150, 01.05.2017, p. 214-223.

Research output: Contribution to journalArticle

@article{08a609658d804df29a499a58aed56788,
title = "Non-toxic and clean crosslinking system for protein materials: Effect of extenders on crosslinking performance",
abstract = "One of the biggest disadvantages of protein cross-linking method is the toxicity of cross-linking agents. In this paper, a clean and non-toxic cross-linking system for protein materials using citric acid (CA) as crosslinker and polyols as cross-linking extender was established. Incorporation of polyols could enhance cross-linking performance of CA by forming branched network among protein molecules. Effect of polyol structure on the cross-linking performance was studied systematically, and keratin fiber was used as an example of protein materials. Extenders containing both primary and secondary hydroxyl groups were more effective in enhancing cross-linking efficiency. Changes in the amounts of carboxyl group, amine group and sulfhydryl group of keratin fiber after cross-linking were measured via potentiometric and conductometric titrations to quantify the cross-linking efficiency. Cross-linking mechanism was also illustrated. With proper numbers of primary hydroxyl groups, secondary hydroxyl groups and backbone length, polyols could be efficient in enhancing performance of this cross-linking system. This novel clean cross-linking system could be used for not only protein engineering, but also other areas where utilization of cross-linking agents are contemplated.",
keywords = "Citric acid, Keratin fiber, Non-toxic cross-linking, Polyol",
author = "Kaili Song and Helan Xu and Bingnan Mu and Kongliang Xie and Yiqi Yang",
year = "2017",
month = "5",
day = "1",
doi = "10.1016/j.jclepro.2017.03.025",
language = "English (US)",
volume = "150",
pages = "214--223",
journal = "Journal of Cleaner Production",
issn = "0959-6526",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Non-toxic and clean crosslinking system for protein materials

T2 - Effect of extenders on crosslinking performance

AU - Song, Kaili

AU - Xu, Helan

AU - Mu, Bingnan

AU - Xie, Kongliang

AU - Yang, Yiqi

PY - 2017/5/1

Y1 - 2017/5/1

N2 - One of the biggest disadvantages of protein cross-linking method is the toxicity of cross-linking agents. In this paper, a clean and non-toxic cross-linking system for protein materials using citric acid (CA) as crosslinker and polyols as cross-linking extender was established. Incorporation of polyols could enhance cross-linking performance of CA by forming branched network among protein molecules. Effect of polyol structure on the cross-linking performance was studied systematically, and keratin fiber was used as an example of protein materials. Extenders containing both primary and secondary hydroxyl groups were more effective in enhancing cross-linking efficiency. Changes in the amounts of carboxyl group, amine group and sulfhydryl group of keratin fiber after cross-linking were measured via potentiometric and conductometric titrations to quantify the cross-linking efficiency. Cross-linking mechanism was also illustrated. With proper numbers of primary hydroxyl groups, secondary hydroxyl groups and backbone length, polyols could be efficient in enhancing performance of this cross-linking system. This novel clean cross-linking system could be used for not only protein engineering, but also other areas where utilization of cross-linking agents are contemplated.

AB - One of the biggest disadvantages of protein cross-linking method is the toxicity of cross-linking agents. In this paper, a clean and non-toxic cross-linking system for protein materials using citric acid (CA) as crosslinker and polyols as cross-linking extender was established. Incorporation of polyols could enhance cross-linking performance of CA by forming branched network among protein molecules. Effect of polyol structure on the cross-linking performance was studied systematically, and keratin fiber was used as an example of protein materials. Extenders containing both primary and secondary hydroxyl groups were more effective in enhancing cross-linking efficiency. Changes in the amounts of carboxyl group, amine group and sulfhydryl group of keratin fiber after cross-linking were measured via potentiometric and conductometric titrations to quantify the cross-linking efficiency. Cross-linking mechanism was also illustrated. With proper numbers of primary hydroxyl groups, secondary hydroxyl groups and backbone length, polyols could be efficient in enhancing performance of this cross-linking system. This novel clean cross-linking system could be used for not only protein engineering, but also other areas where utilization of cross-linking agents are contemplated.

KW - Citric acid

KW - Keratin fiber

KW - Non-toxic cross-linking

KW - Polyol

UR - http://www.scopus.com/inward/record.url?scp=85016017747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016017747&partnerID=8YFLogxK

U2 - 10.1016/j.jclepro.2017.03.025

DO - 10.1016/j.jclepro.2017.03.025

M3 - Article

AN - SCOPUS:85016017747

VL - 150

SP - 214

EP - 223

JO - Journal of Cleaner Production

JF - Journal of Cleaner Production

SN - 0959-6526

ER -