Non-specific binding of anti-human IgE peroxidase-linked conjugates to legume proteins in immunoblots

Anne M. Herian, Stephen L Taylor

Research output: Contribution to journalArticle

Abstract

Non-specific binding was observed in immunoblotting of legume proteins with commercial anti-human IgE and IgG antibodies conjugated with peroxidase when incubations were conducted without prior incubation with human sera of legume-allergic individuals. The non-specific binding was encountered with ten legume species. It was not associated with endogenous peroxidase or the peanut or soybean lectins. The degree of non-specific binding diminished with roasted peanut extracts as compared to raw peanut extracts. The cause of this non-specific binding remains unknown, although it may be related to the animal source of the antiserum or the method of conjugation of the antibody with peroxidase. This interference in immunoblotting can be easily detected with controls lacking incubation with human sera.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalJournal of Immunological Methods
Volume140
Issue number2
DOIs
StatePublished - Jan 1 1991

Fingerprint

Fabaceae
Peroxidase
Immunoblotting
Peanut Agglutinin
Proteins
Antibodies
Serum
Immune Sera
anti-IgE antibodies
Arachis
anti-IgG
soybean lectin

Keywords

  • (Legume)
  • Allergy
  • Food allergy
  • IgE
  • Immunoblotting

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Non-specific binding of anti-human IgE peroxidase-linked conjugates to legume proteins in immunoblots. / Herian, Anne M.; Taylor, Stephen L.

In: Journal of Immunological Methods, Vol. 140, No. 2, 01.01.1991, p. 153-158.

Research output: Contribution to journalArticle

@article{51a52059c8754e37b7f41e06cae79fc4,
title = "Non-specific binding of anti-human IgE peroxidase-linked conjugates to legume proteins in immunoblots",
abstract = "Non-specific binding was observed in immunoblotting of legume proteins with commercial anti-human IgE and IgG antibodies conjugated with peroxidase when incubations were conducted without prior incubation with human sera of legume-allergic individuals. The non-specific binding was encountered with ten legume species. It was not associated with endogenous peroxidase or the peanut or soybean lectins. The degree of non-specific binding diminished with roasted peanut extracts as compared to raw peanut extracts. The cause of this non-specific binding remains unknown, although it may be related to the animal source of the antiserum or the method of conjugation of the antibody with peroxidase. This interference in immunoblotting can be easily detected with controls lacking incubation with human sera.",
keywords = "(Legume), Allergy, Food allergy, IgE, Immunoblotting",
author = "Herian, {Anne M.} and Taylor, {Stephen L}",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0022-1759(91)90365-M",
language = "English (US)",
volume = "140",
pages = "153--158",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Non-specific binding of anti-human IgE peroxidase-linked conjugates to legume proteins in immunoblots

AU - Herian, Anne M.

AU - Taylor, Stephen L

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Non-specific binding was observed in immunoblotting of legume proteins with commercial anti-human IgE and IgG antibodies conjugated with peroxidase when incubations were conducted without prior incubation with human sera of legume-allergic individuals. The non-specific binding was encountered with ten legume species. It was not associated with endogenous peroxidase or the peanut or soybean lectins. The degree of non-specific binding diminished with roasted peanut extracts as compared to raw peanut extracts. The cause of this non-specific binding remains unknown, although it may be related to the animal source of the antiserum or the method of conjugation of the antibody with peroxidase. This interference in immunoblotting can be easily detected with controls lacking incubation with human sera.

AB - Non-specific binding was observed in immunoblotting of legume proteins with commercial anti-human IgE and IgG antibodies conjugated with peroxidase when incubations were conducted without prior incubation with human sera of legume-allergic individuals. The non-specific binding was encountered with ten legume species. It was not associated with endogenous peroxidase or the peanut or soybean lectins. The degree of non-specific binding diminished with roasted peanut extracts as compared to raw peanut extracts. The cause of this non-specific binding remains unknown, although it may be related to the animal source of the antiserum or the method of conjugation of the antibody with peroxidase. This interference in immunoblotting can be easily detected with controls lacking incubation with human sera.

KW - (Legume)

KW - Allergy

KW - Food allergy

KW - IgE

KW - Immunoblotting

UR - http://www.scopus.com/inward/record.url?scp=0025870612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025870612&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(91)90365-M

DO - 10.1016/0022-1759(91)90365-M

M3 - Article

VL - 140

SP - 153

EP - 158

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 2

ER -