Non-mitogenic human acidic fibroblast growth factor reduces retinal degeneration induced by sodium iodate

Weiwei Chen, Man Yu, Yun Wang, Youwei Peng, Xiaokun Li, Dominic Man Kit Lam, Xiaoming Chen, Xuyang Liu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: To investigate the protective effects of non-mitogenic human acidic fibroblast growth factor on retinal degeneration induced by NaIO 3 in rats. Methods: Retinal degeneration was induced in adult male Wistar rats via caudal-vein injection of 1% NaIO3 at 50 mg/kg. One h after NaIO3 treatment, the right eyes received intravitreal injection of 2.5 μg nm-haFGF in 10 μL saline and the left eyes received saline alone as vehicle-treated eyes. Retinal function in rats was evaluated by electroretinogram (ERG) before injection and 1, 7, and 21 days postinjection. Additional rat eyes were enucleated 7 and 21 days postinjection, fixed, and processed for histological examination. Results: A model of retinal degeneration in rat was established successfully using NaIO3 injection. Significant decreases in both ERG a- and b-wave amplitudes were detected in NaIO3-injected rats when compared with the normal animals (P < 0.05) on day 7 postinjection. Importantly, at the seventh day after intravitreal nm-haFGF treatment on NaIO3-injected rats, the nm-haFGF-treated eyes showed a significant improvement in the ERG amplitudes of both a- and b-waves when compared with vehicle-treated eyes (P < 0.05). In addition, the disruptions of photoreceptor outer segments and the retinal pigment epithelium monolayer were much less frequently observed in the nm-haFGF-treated eyes than the vehicle-treated eyes, and the outer nuclear layer thickness in the nm-haFGF-treated eyes was similar to that of the normal eyes. Conclusions: Intravitreal delivery of nm-haFGF appears to have neuroprotective effect on retinal degeneration induced by NaIO3.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalJournal of Ocular Pharmacology and Therapeutics
Volume25
Issue number4
DOIs
StatePublished - Aug 1 2009

Fingerprint

Fibroblast Growth Factor 1
Retinal Degeneration
Injections
sodium iodate
Intravitreal Injections
Retinal Pigment Epithelium
Neuroprotective Agents
Wistar Rats
Veins

ASJC Scopus subject areas

  • Ophthalmology
  • Pharmacology
  • Pharmacology (medical)

Cite this

Non-mitogenic human acidic fibroblast growth factor reduces retinal degeneration induced by sodium iodate. / Chen, Weiwei; Yu, Man; Wang, Yun; Peng, Youwei; Li, Xiaokun; Lam, Dominic Man Kit; Chen, Xiaoming; Liu, Xuyang.

In: Journal of Ocular Pharmacology and Therapeutics, Vol. 25, No. 4, 01.08.2009, p. 315-319.

Research output: Contribution to journalArticle

Chen, Weiwei ; Yu, Man ; Wang, Yun ; Peng, Youwei ; Li, Xiaokun ; Lam, Dominic Man Kit ; Chen, Xiaoming ; Liu, Xuyang. / Non-mitogenic human acidic fibroblast growth factor reduces retinal degeneration induced by sodium iodate. In: Journal of Ocular Pharmacology and Therapeutics. 2009 ; Vol. 25, No. 4. pp. 315-319.
@article{90da3f842caa4fc0845ab04c4e4b3132,
title = "Non-mitogenic human acidic fibroblast growth factor reduces retinal degeneration induced by sodium iodate",
abstract = "Purpose: To investigate the protective effects of non-mitogenic human acidic fibroblast growth factor on retinal degeneration induced by NaIO 3 in rats. Methods: Retinal degeneration was induced in adult male Wistar rats via caudal-vein injection of 1{\%} NaIO3 at 50 mg/kg. One h after NaIO3 treatment, the right eyes received intravitreal injection of 2.5 μg nm-haFGF in 10 μL saline and the left eyes received saline alone as vehicle-treated eyes. Retinal function in rats was evaluated by electroretinogram (ERG) before injection and 1, 7, and 21 days postinjection. Additional rat eyes were enucleated 7 and 21 days postinjection, fixed, and processed for histological examination. Results: A model of retinal degeneration in rat was established successfully using NaIO3 injection. Significant decreases in both ERG a- and b-wave amplitudes were detected in NaIO3-injected rats when compared with the normal animals (P < 0.05) on day 7 postinjection. Importantly, at the seventh day after intravitreal nm-haFGF treatment on NaIO3-injected rats, the nm-haFGF-treated eyes showed a significant improvement in the ERG amplitudes of both a- and b-waves when compared with vehicle-treated eyes (P < 0.05). In addition, the disruptions of photoreceptor outer segments and the retinal pigment epithelium monolayer were much less frequently observed in the nm-haFGF-treated eyes than the vehicle-treated eyes, and the outer nuclear layer thickness in the nm-haFGF-treated eyes was similar to that of the normal eyes. Conclusions: Intravitreal delivery of nm-haFGF appears to have neuroprotective effect on retinal degeneration induced by NaIO3.",
author = "Weiwei Chen and Man Yu and Yun Wang and Youwei Peng and Xiaokun Li and Lam, {Dominic Man Kit} and Xiaoming Chen and Xuyang Liu",
year = "2009",
month = "8",
day = "1",
doi = "10.1089/jop.2009.0015",
language = "English (US)",
volume = "25",
pages = "315--319",
journal = "Journal of Ocular Pharmacology and Therapeutics",
issn = "1080-7683",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Non-mitogenic human acidic fibroblast growth factor reduces retinal degeneration induced by sodium iodate

AU - Chen, Weiwei

AU - Yu, Man

AU - Wang, Yun

AU - Peng, Youwei

AU - Li, Xiaokun

AU - Lam, Dominic Man Kit

AU - Chen, Xiaoming

AU - Liu, Xuyang

PY - 2009/8/1

Y1 - 2009/8/1

N2 - Purpose: To investigate the protective effects of non-mitogenic human acidic fibroblast growth factor on retinal degeneration induced by NaIO 3 in rats. Methods: Retinal degeneration was induced in adult male Wistar rats via caudal-vein injection of 1% NaIO3 at 50 mg/kg. One h after NaIO3 treatment, the right eyes received intravitreal injection of 2.5 μg nm-haFGF in 10 μL saline and the left eyes received saline alone as vehicle-treated eyes. Retinal function in rats was evaluated by electroretinogram (ERG) before injection and 1, 7, and 21 days postinjection. Additional rat eyes were enucleated 7 and 21 days postinjection, fixed, and processed for histological examination. Results: A model of retinal degeneration in rat was established successfully using NaIO3 injection. Significant decreases in both ERG a- and b-wave amplitudes were detected in NaIO3-injected rats when compared with the normal animals (P < 0.05) on day 7 postinjection. Importantly, at the seventh day after intravitreal nm-haFGF treatment on NaIO3-injected rats, the nm-haFGF-treated eyes showed a significant improvement in the ERG amplitudes of both a- and b-waves when compared with vehicle-treated eyes (P < 0.05). In addition, the disruptions of photoreceptor outer segments and the retinal pigment epithelium monolayer were much less frequently observed in the nm-haFGF-treated eyes than the vehicle-treated eyes, and the outer nuclear layer thickness in the nm-haFGF-treated eyes was similar to that of the normal eyes. Conclusions: Intravitreal delivery of nm-haFGF appears to have neuroprotective effect on retinal degeneration induced by NaIO3.

AB - Purpose: To investigate the protective effects of non-mitogenic human acidic fibroblast growth factor on retinal degeneration induced by NaIO 3 in rats. Methods: Retinal degeneration was induced in adult male Wistar rats via caudal-vein injection of 1% NaIO3 at 50 mg/kg. One h after NaIO3 treatment, the right eyes received intravitreal injection of 2.5 μg nm-haFGF in 10 μL saline and the left eyes received saline alone as vehicle-treated eyes. Retinal function in rats was evaluated by electroretinogram (ERG) before injection and 1, 7, and 21 days postinjection. Additional rat eyes were enucleated 7 and 21 days postinjection, fixed, and processed for histological examination. Results: A model of retinal degeneration in rat was established successfully using NaIO3 injection. Significant decreases in both ERG a- and b-wave amplitudes were detected in NaIO3-injected rats when compared with the normal animals (P < 0.05) on day 7 postinjection. Importantly, at the seventh day after intravitreal nm-haFGF treatment on NaIO3-injected rats, the nm-haFGF-treated eyes showed a significant improvement in the ERG amplitudes of both a- and b-waves when compared with vehicle-treated eyes (P < 0.05). In addition, the disruptions of photoreceptor outer segments and the retinal pigment epithelium monolayer were much less frequently observed in the nm-haFGF-treated eyes than the vehicle-treated eyes, and the outer nuclear layer thickness in the nm-haFGF-treated eyes was similar to that of the normal eyes. Conclusions: Intravitreal delivery of nm-haFGF appears to have neuroprotective effect on retinal degeneration induced by NaIO3.

UR - http://www.scopus.com/inward/record.url?scp=68549112869&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68549112869&partnerID=8YFLogxK

U2 - 10.1089/jop.2009.0015

DO - 10.1089/jop.2009.0015

M3 - Article

C2 - 19650706

AN - SCOPUS:68549112869

VL - 25

SP - 315

EP - 319

JO - Journal of Ocular Pharmacology and Therapeutics

JF - Journal of Ocular Pharmacology and Therapeutics

SN - 1080-7683

IS - 4

ER -