Non-invasive measurement of effective diffusivities in cell immobilization gels through use of near-infrared spectroscopy

Brenna L. Frazier, Paul Larmour, Mark R. Riley

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Encapsulated cell systems provide some advantages over typical suspension cell cultivations as higher cell densities may be obtained; however, the supply of nutrients to the cells often is a limiting factor in productivity. In this study, we describe the development of a new approach to characterize the effective diffusivity of nutrients in immobilized cell materials. Near-infrared spectroscopy is employed to measure nutrient concentrations within a specially designed diffusion chamber that permits noninvasive sampling at ten spatial positions and multiple timepoints. To demonstrate this technique, we measured the effective diffusivity of glutamine in a cell-free 3% (w/w) agarose gel and determined the effective diffusivity (Deff) = 6.46 × 10-10 m2/s, which is in good agreement with theoretical values.

Original languageEnglish (US)
Pages (from-to)364-368
Number of pages5
JournalBiotechnology and Bioengineering
Volume72
Issue number3
DOIs
StatePublished - Feb 5 2001

Fingerprint

Cell immobilization
Near infrared spectroscopy
Near-Infrared Spectroscopy
Immobilization
Gels
Nutrients
Food
Immobilized Cells
Glutamine
Sepharose
Suspensions
Cell Count
Productivity
Cells
Sampling

Keywords

  • Diffusion
  • Immobilized cells
  • Near-infrared (NIR) spectroscopy
  • Noninvasive measurement

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Non-invasive measurement of effective diffusivities in cell immobilization gels through use of near-infrared spectroscopy. / Frazier, Brenna L.; Larmour, Paul; Riley, Mark R.

In: Biotechnology and Bioengineering, Vol. 72, No. 3, 05.02.2001, p. 364-368.

Research output: Contribution to journalArticle

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