Non-destructive determination of maize leaf and canopy chlorophyll content

Verónica Ciganda, Anatoly Gitelson, James Schepers

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

The objective of this study was to develop a rapid non-destructive technique to estimate total chlorophyll (Chl) content in a maize canopy using Chl content in a single leaf. The approach was (1) to calibrate and validate a reflectance-based non-destructive technique to estimate leaf Chl in maize; (2) to quantify the relative contribution of each leaf Chl to the total Chl in the canopy; and (3) to establish a relationship between leaf Chl content and total Chl in a maize canopy. The Red Edge Chlorophyll Index CIred edge=(RNIR/Rred edge)-1 based on reflectances, R, in the red edge (720-730 nm) and near infrared (770-800 nm) was found to be an accurate measure of maize leaf Chl. It was able to predict leaf Chl ranging from 10 to 805 mg Chl m-2 with root mean-square error less than 38 mg Chl m-2. Relationships between Chl content in each maize leaf and total canopy Chl content were established and showed that Chl in the collar leaf before silking or ear leaves explained more than 80% and 87% of the variation in total Chl in a maize canopy, respectively. Thus, non-destructive measurements of both reflectance and area of a single leaf (either collar or ear) can be used to accurately estimate total Chl content in a maize canopy.

Original languageEnglish (US)
Pages (from-to)157-167
Number of pages11
JournalJournal of Plant Physiology
Volume166
Issue number2
DOIs
StatePublished - Jan 30 2009

Fingerprint

Chlorophyll
Zea mays
canopy
chlorophyll
corn
leaves
reflectance
collars
Ear
ears

Keywords

  • Chlorophyll
  • Maize
  • Model
  • Non-destructive
  • Reflectance

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Non-destructive determination of maize leaf and canopy chlorophyll content. / Ciganda, Verónica; Gitelson, Anatoly; Schepers, James.

In: Journal of Plant Physiology, Vol. 166, No. 2, 30.01.2009, p. 157-167.

Research output: Contribution to journalArticle

Ciganda, Verónica ; Gitelson, Anatoly ; Schepers, James. / Non-destructive determination of maize leaf and canopy chlorophyll content. In: Journal of Plant Physiology. 2009 ; Vol. 166, No. 2. pp. 157-167.
@article{6a31e9c2d20a47bea519ed0b8b7d174f,
title = "Non-destructive determination of maize leaf and canopy chlorophyll content",
abstract = "The objective of this study was to develop a rapid non-destructive technique to estimate total chlorophyll (Chl) content in a maize canopy using Chl content in a single leaf. The approach was (1) to calibrate and validate a reflectance-based non-destructive technique to estimate leaf Chl in maize; (2) to quantify the relative contribution of each leaf Chl to the total Chl in the canopy; and (3) to establish a relationship between leaf Chl content and total Chl in a maize canopy. The Red Edge Chlorophyll Index CIred edge=(RNIR/Rred edge)-1 based on reflectances, R, in the red edge (720-730 nm) and near infrared (770-800 nm) was found to be an accurate measure of maize leaf Chl. It was able to predict leaf Chl ranging from 10 to 805 mg Chl m-2 with root mean-square error less than 38 mg Chl m-2. Relationships between Chl content in each maize leaf and total canopy Chl content were established and showed that Chl in the collar leaf before silking or ear leaves explained more than 80{\%} and 87{\%} of the variation in total Chl in a maize canopy, respectively. Thus, non-destructive measurements of both reflectance and area of a single leaf (either collar or ear) can be used to accurately estimate total Chl content in a maize canopy.",
keywords = "Chlorophyll, Maize, Model, Non-destructive, Reflectance",
author = "Ver{\'o}nica Ciganda and Anatoly Gitelson and James Schepers",
year = "2009",
month = "1",
day = "30",
doi = "10.1016/j.jplph.2008.03.004",
language = "English (US)",
volume = "166",
pages = "157--167",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "2",

}

TY - JOUR

T1 - Non-destructive determination of maize leaf and canopy chlorophyll content

AU - Ciganda, Verónica

AU - Gitelson, Anatoly

AU - Schepers, James

PY - 2009/1/30

Y1 - 2009/1/30

N2 - The objective of this study was to develop a rapid non-destructive technique to estimate total chlorophyll (Chl) content in a maize canopy using Chl content in a single leaf. The approach was (1) to calibrate and validate a reflectance-based non-destructive technique to estimate leaf Chl in maize; (2) to quantify the relative contribution of each leaf Chl to the total Chl in the canopy; and (3) to establish a relationship between leaf Chl content and total Chl in a maize canopy. The Red Edge Chlorophyll Index CIred edge=(RNIR/Rred edge)-1 based on reflectances, R, in the red edge (720-730 nm) and near infrared (770-800 nm) was found to be an accurate measure of maize leaf Chl. It was able to predict leaf Chl ranging from 10 to 805 mg Chl m-2 with root mean-square error less than 38 mg Chl m-2. Relationships between Chl content in each maize leaf and total canopy Chl content were established and showed that Chl in the collar leaf before silking or ear leaves explained more than 80% and 87% of the variation in total Chl in a maize canopy, respectively. Thus, non-destructive measurements of both reflectance and area of a single leaf (either collar or ear) can be used to accurately estimate total Chl content in a maize canopy.

AB - The objective of this study was to develop a rapid non-destructive technique to estimate total chlorophyll (Chl) content in a maize canopy using Chl content in a single leaf. The approach was (1) to calibrate and validate a reflectance-based non-destructive technique to estimate leaf Chl in maize; (2) to quantify the relative contribution of each leaf Chl to the total Chl in the canopy; and (3) to establish a relationship between leaf Chl content and total Chl in a maize canopy. The Red Edge Chlorophyll Index CIred edge=(RNIR/Rred edge)-1 based on reflectances, R, in the red edge (720-730 nm) and near infrared (770-800 nm) was found to be an accurate measure of maize leaf Chl. It was able to predict leaf Chl ranging from 10 to 805 mg Chl m-2 with root mean-square error less than 38 mg Chl m-2. Relationships between Chl content in each maize leaf and total canopy Chl content were established and showed that Chl in the collar leaf before silking or ear leaves explained more than 80% and 87% of the variation in total Chl in a maize canopy, respectively. Thus, non-destructive measurements of both reflectance and area of a single leaf (either collar or ear) can be used to accurately estimate total Chl content in a maize canopy.

KW - Chlorophyll

KW - Maize

KW - Model

KW - Non-destructive

KW - Reflectance

UR - http://www.scopus.com/inward/record.url?scp=57449111977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57449111977&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2008.03.004

DO - 10.1016/j.jplph.2008.03.004

M3 - Article

C2 - 18541334

AN - SCOPUS:57449111977

VL - 166

SP - 157

EP - 167

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 2

ER -