Non-contact method for directing electrotaxis

Dinesh K. Ahirwar, Mohd W. Nasser, Travis H. Jones, Emily K. Sequin, Joseph D. West, Timothy L. Henthorne, Joshua Javor, Aniruddha M. Kaushik, Ramesh K. Ganju, Vish V. Subramaniam

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We present a method to induce electric fields and drive electrotaxis (galvanotaxis) without the need for electrodes to be in contact with the media containing the cell cultures. We report experimental results using a modification of the transmembrane assay, demonstrating the hindrance of migration of breast cancer cells (SCP2) when an induced a.c. electric field is present in the appropriate direction (i.e. in the direction of migration). Of significance is that migration of these cells is hindered at electric field strengths many orders of magnitude (5 to 6) below those previously reported for d.c. electrotaxis, and even in the presence of a chemokine (SDF-1α) or a growth factor (EGF). Induced a.c. electric fields applied in the direction of migration are also shown to hinder motility of non-transformed human mammary epithelial cells (MCF10A) in the presence of the growth factor EGF. In addition, we also show how our method can be applied to other cell migration assays (scratch assay), and by changing the coil design and holder, that it is also compatible with commercially available multi-well culture plates.

Original languageEnglish (US)
Article number11005
JournalScientific reports
Volume5
DOIs
StatePublished - Jun 9 2015

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Epidermal Growth Factor
Intercellular Signaling Peptides and Proteins
Cell Migration Assays
Chemokines
Cell Movement
Electrodes
Breast
Cell Culture Techniques
Epithelial Cells
Breast Neoplasms
Direction compound
Drive
Taxis Response

ASJC Scopus subject areas

  • General

Cite this

Ahirwar, D. K., Nasser, M. W., Jones, T. H., Sequin, E. K., West, J. D., Henthorne, T. L., ... Subramaniam, V. V. (2015). Non-contact method for directing electrotaxis. Scientific reports, 5, [11005]. https://doi.org/10.1038/srep11005

Non-contact method for directing electrotaxis. / Ahirwar, Dinesh K.; Nasser, Mohd W.; Jones, Travis H.; Sequin, Emily K.; West, Joseph D.; Henthorne, Timothy L.; Javor, Joshua; Kaushik, Aniruddha M.; Ganju, Ramesh K.; Subramaniam, Vish V.

In: Scientific reports, Vol. 5, 11005, 09.06.2015.

Research output: Contribution to journalArticle

Ahirwar, DK, Nasser, MW, Jones, TH, Sequin, EK, West, JD, Henthorne, TL, Javor, J, Kaushik, AM, Ganju, RK & Subramaniam, VV 2015, 'Non-contact method for directing electrotaxis', Scientific reports, vol. 5, 11005. https://doi.org/10.1038/srep11005
Ahirwar DK, Nasser MW, Jones TH, Sequin EK, West JD, Henthorne TL et al. Non-contact method for directing electrotaxis. Scientific reports. 2015 Jun 9;5. 11005. https://doi.org/10.1038/srep11005
Ahirwar, Dinesh K. ; Nasser, Mohd W. ; Jones, Travis H. ; Sequin, Emily K. ; West, Joseph D. ; Henthorne, Timothy L. ; Javor, Joshua ; Kaushik, Aniruddha M. ; Ganju, Ramesh K. ; Subramaniam, Vish V. / Non-contact method for directing electrotaxis. In: Scientific reports. 2015 ; Vol. 5.
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