NMR metabolic profiling of Aspergillus nidulans to monitor drug and protein activity

Paxton Forgue, Steven Halouska, Mark Werth, Kaimei Xu, Steve Harris, Robert Powers

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We describe a general protocol for using comparative NMR metabolomics data to infer in vivo efficacy, specificity and toxicity of chemical leads within a drug discovery program. The methodology is demonstrated using Aspergillus nidulans to monitor the activity of urate oxidase and orotidine-5′- phosphate decarboxylase and the impact of 8-azaxanthine, an inhibitor of urate oxidase. 8-azaxanthine is shown to inhibit A. nidulans hyphal growth by in vivo inactivation of urate oxidase.

Original languageEnglish (US)
Pages (from-to)1916-1923
Number of pages8
JournalJournal of proteome research
Volume5
Issue number8
DOIs
StatePublished - Aug 1 2006

Fingerprint

Urate Oxidase
Aspergillus nidulans
Aspergillus
Nuclear magnetic resonance
Orotidine-5'-Phosphate Decarboxylase
Pharmaceutical Preparations
Proteins
Metabolomics
Drug Discovery
Toxicity
Growth
8-azaxanthine

Keywords

  • Aspergillus nidulans
  • Drug discovery
  • Metabolomics
  • NMR
  • Principle component analysis
  • Purine degradation pathway
  • Pyrimidine biosynthetic pathway

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

NMR metabolic profiling of Aspergillus nidulans to monitor drug and protein activity. / Forgue, Paxton; Halouska, Steven; Werth, Mark; Xu, Kaimei; Harris, Steve; Powers, Robert.

In: Journal of proteome research, Vol. 5, No. 8, 01.08.2006, p. 1916-1923.

Research output: Contribution to journalArticle

Forgue, Paxton ; Halouska, Steven ; Werth, Mark ; Xu, Kaimei ; Harris, Steve ; Powers, Robert. / NMR metabolic profiling of Aspergillus nidulans to monitor drug and protein activity. In: Journal of proteome research. 2006 ; Vol. 5, No. 8. pp. 1916-1923.
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