NMR analysis of a stress response metabolic signaling network

Bo Zhang, Steven Halouska, Charles E. Schiaffo, Marat R. Sadykov, Greg A Somerville, Robert Powers

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We previously hypothesized that Staphylococcus epidermidis senses a diverse set of environmental and nutritional factors associated with biofilm formation through a modulation in the activity of the tricarboxylic acid (TCA) cycle. Herein, we report our further investigation of the impact of additional environmental stress factors on TCA cycle activity and provide a detailed description of our NMR methodology. S. epidermidis wild-type strain 1457 was treated with stressors that are associated with biofilm formation, a sublethal dose of tetracycline, 5% NaCl, 2% glucose, and autoinducer-2 (AI-2). As controls and to integrate our current data with our previous study, 4% ethanol stress and iron-limitation were also used. Consistent with our prior observations, the effect of many environmental stress factors on the S. epidermidis metabolome was essentially identical to the effect of TCA cycle inactivation in the aconitase mutant strain 1457-acnA::tetM. A detailed quantitative analysis of metabolite concentration changes using 2D 1H- 13C HSQC and 1H- 1H TOCSY spectra identified a network of 37 metabolites uniformly affected by the stressors and TCA cycle inactivation. We postulate that the TCA cycle acts as the central pathway in a metabolic signaling network.

Original languageEnglish (US)
Pages (from-to)3743-3754
Number of pages12
JournalJournal of Proteome Research
Volume10
Issue number8
DOIs
StatePublished - Aug 5 2011

Fingerprint

Citric Acid Cycle
Metabolic Networks and Pathways
Nuclear magnetic resonance
Staphylococcus epidermidis
Biofilms
Metabolites
Aconitate Hydratase
Metabolome
Tetracycline
Ethanol
Iron
Modulation
Glucose
Chemical analysis

Keywords

  • NMR
  • bioinformatics
  • central metabolism
  • metabolomics
  • stress response

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

NMR analysis of a stress response metabolic signaling network. / Zhang, Bo; Halouska, Steven; Schiaffo, Charles E.; Sadykov, Marat R.; Somerville, Greg A; Powers, Robert.

In: Journal of Proteome Research, Vol. 10, No. 8, 05.08.2011, p. 3743-3754.

Research output: Contribution to journalArticle

Zhang, Bo ; Halouska, Steven ; Schiaffo, Charles E. ; Sadykov, Marat R. ; Somerville, Greg A ; Powers, Robert. / NMR analysis of a stress response metabolic signaling network. In: Journal of Proteome Research. 2011 ; Vol. 10, No. 8. pp. 3743-3754.
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