Nitrous oxide produces antinociceptive response via α(2B) and/or α(2C) adrenoceptor subtypes in mice

Tian Zhi Guo, M. Frances Davies, Wade S. Kingery, Andrew J Patterson, Lee E. Limbird, Mervyn Maze

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Background. Opiate receptors in the periaqueductal gray region and α2 adrenoceptors in the spinal cord of the rat mediate the antinociceptive properties of nitrous oxide (N2O). The availability of genetically altered mice facilitates the detection of the precise protein species involved in the transduction pathway. In this study, the authors establish the similarity between rats and mice in the antinociceptive action of N2O and investigate which α2 adrenoceptor subtypes mediate this response. Methods: After obtaining institutional approval, antinociceptive dose-response and time- course to N2O was measured in wild-type and transgenic mice (D79N), with a nonfunctional α(2A) adrenoceptor using tail-flick latency. The antinociceptive effect of N2O was tested after pretreatment systemically with yohimbine (nonselective α2 antagonist), naloxone (opiate antagonist), L659,066 (peripheral α2-antagonist) and prazosin (α(2B)- and α(2C)- selective antagonist). The tail-flick latency to dexmedetomidine (D-med), a nonselective α2 agonist, was tested in wild-type and transgenic mice. Results: N2O produced antinociception in both D79N transgenic and wild-type litter mates, although the response was less pronounced in the transgenic mice. Antinociception from N2O decreased over time with continuing exposure, and the decrement was more pronounced in the transgenic mice. The antinociceptive response could be dose dependently antagonized by opiate receptor and selective α(2B)-/α(2C)-receptor antagonists but not by a central nervous system-impermeant α2 antagonist (L659,066). Whereas dexmedetomidine exhibited no antinociceptive response in the D79N mice, the robust antinociceptive response in the wild-type litter mates could not be blocked by a selective α(2B)-/α(2C)-receptor antagonist. Conclusion. These data confirm that the antinociceptive response to an exogenous α2-agonist is mediated by an α(2A) adrenoceptor and that there appears to be a role for the α(2B)- or α(2C)-adrenoceptor subtypes, or both, in the analgesic response to N2O.

Original languageEnglish (US)
Pages (from-to)470-476
Number of pages7
JournalAnesthesiology
Volume90
Issue number2
DOIs
StatePublished - Feb 1 1999

Fingerprint

Nitrous Oxide
Adrenergic Receptors
L 659066
Transgenic Mice
Dexmedetomidine
Opioid Receptors
Tail
Opiate Alkaloids
Periaqueductal Gray
Yohimbine
Prazosin
Naloxone
Reaction Time
Analgesics
Spinal Cord
Central Nervous System
Proteins

Keywords

  • Analgesia
  • Opiate receptors
  • Tail flick

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Guo, T. Z., Davies, M. F., Kingery, W. S., Patterson, A. J., Limbird, L. E., & Maze, M. (1999). Nitrous oxide produces antinociceptive response via α(2B) and/or α(2C) adrenoceptor subtypes in mice. Anesthesiology, 90(2), 470-476. https://doi.org/10.1097/00000542-199902000-00022

Nitrous oxide produces antinociceptive response via α(2B) and/or α(2C) adrenoceptor subtypes in mice. / Guo, Tian Zhi; Davies, M. Frances; Kingery, Wade S.; Patterson, Andrew J; Limbird, Lee E.; Maze, Mervyn.

In: Anesthesiology, Vol. 90, No. 2, 01.02.1999, p. 470-476.

Research output: Contribution to journalArticle

Guo, TZ, Davies, MF, Kingery, WS, Patterson, AJ, Limbird, LE & Maze, M 1999, 'Nitrous oxide produces antinociceptive response via α(2B) and/or α(2C) adrenoceptor subtypes in mice', Anesthesiology, vol. 90, no. 2, pp. 470-476. https://doi.org/10.1097/00000542-199902000-00022
Guo, Tian Zhi ; Davies, M. Frances ; Kingery, Wade S. ; Patterson, Andrew J ; Limbird, Lee E. ; Maze, Mervyn. / Nitrous oxide produces antinociceptive response via α(2B) and/or α(2C) adrenoceptor subtypes in mice. In: Anesthesiology. 1999 ; Vol. 90, No. 2. pp. 470-476.
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