Nitric oxide synthase-dependent responses of the basilar artery during acute infusion of nicotine

William G. Mayhan, Denise M. Arrick, Glenda M. Sharpe, Hong Sun

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Introduction: Our goals were to determine whether acute exposure to nicotine alters nitric oxide synthase (NOS)-dependent responses of the basilar artery and to identify a potential role for activation of NAD(P)H oxidase in nicotine-induced impairment in NOS-dependent responses of the basilar artery. Methods: We measured in vivo diameter of the basilar artery in response to NOS-dependent (acetylcholine) and NOS-independent (nitroglycerin) agonists before and during an acute infusion of nicotine (2 μg/kg/min intravenously for 30 min followed by a maintenance dose of 0.35 μg/kg/ min). In addition, we measured superoxide anion production (lucigenin chemiluminescence) by the basilar artery in response to nicotine in the absence or presence of apocynin. Results: We found that NOS-dependent, but not NOS-independent, vasodilation was impaired during infusion of nicotine. In addition, treatment of the basilar artery with apocynin (100 μM, 30 min prior to infusion of nicotine) prevented nicotine-induced impairment in NOS-dependent vasodilation. Further, the production of superoxide anion was increased in the basilar artery by nicotine, and this increase could be inhibited by apocynin. Discussion: Our findings suggest that acute exposure to nicotine impairs NOS-dependent dilation of the basilar artery by a mechanism that appears to be related to the release of superoxide anion. A possible source of superoxide may be via the activation of NAD(P)H oxidase.

Original languageEnglish (US)
Pages (from-to)270-277
Number of pages8
JournalNicotine and Tobacco Research
Volume11
Issue number3
DOIs
StatePublished - Apr 24 2009

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Basilar Artery
Nicotine
Nitric Oxide Synthase
Superoxides
NADPH Oxidase
Vasodilation
Nitroglycerin
Luminescence
Acetylcholine
Dilatation

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Nitric oxide synthase-dependent responses of the basilar artery during acute infusion of nicotine. / Mayhan, William G.; Arrick, Denise M.; Sharpe, Glenda M.; Sun, Hong.

In: Nicotine and Tobacco Research, Vol. 11, No. 3, 24.04.2009, p. 270-277.

Research output: Contribution to journalArticle

Mayhan, William G. ; Arrick, Denise M. ; Sharpe, Glenda M. ; Sun, Hong. / Nitric oxide synthase-dependent responses of the basilar artery during acute infusion of nicotine. In: Nicotine and Tobacco Research. 2009 ; Vol. 11, No. 3. pp. 270-277.
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