Nitric oxide signaling depends on biotin in Jurkat human lymphoma cells

Rocio Rodriguez-Melendez, Janos Zempleni

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Biotin affects gene expression through a diverse array of cell signaling pathways. Previous studies provided evidence that cGMP-dependent signaling also depends on biotin, but the mechanistic sequence of cGMP regulation by biotin is unknown. Here we tested the hypothesis that the effects of biotin in cGMP-dependent cell signaling are mediated by nitric oxide (NO). Human lymphoid (Jurkat) cells were cultured in media containing deficient (0.025 nmol/L), physiological (0.25 nmol/L), and pharmacological (10 nmol/L) concentrations of biotin for 5 wk. Both levels of intracellular biotin and NO exhibited a dose-dependent relationship in regard to biotin concentrations in culture media. Effects of biotin on NO levels were disrupted by the NO synthase (NOS) inhibitor N-monomethyl-arginine. Biotin-dependent production of NO was linked with biotin-dependent expression of endothelial and neuronal NOS, but not inducible NOS. Previous studies revealed that NO is an activator of guanylate cyclase. Consistent with these previous observations, biotin-dependent generation of NO increased the abundance of cGMP in Jurkat cells. Finally, the biotin-dependent generation of cGMP increased protein kinase G activity. Collectively, the results of this study are consistent with the hypothesis that biotin-dependent cGMP signaling in human lymphoid cells is mediated by NO.

Original languageEnglish (US)
Pages (from-to)429-433
Number of pages5
JournalJournal of Nutrition
Volume139
Issue number3
DOIs
StatePublished - Mar 1 2009

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Biotin
Lymphoma
Nitric Oxide
Nitric Oxide Synthase
Jurkat Cells
Lymphocytes
Cyclic GMP-Dependent Protein Kinases
Guanylate Cyclase
Culture Media
Arginine
Pharmacology

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Nitric oxide signaling depends on biotin in Jurkat human lymphoma cells. / Rodriguez-Melendez, Rocio; Zempleni, Janos.

In: Journal of Nutrition, Vol. 139, No. 3, 01.03.2009, p. 429-433.

Research output: Contribution to journalArticle

Rodriguez-Melendez, Rocio ; Zempleni, Janos. / Nitric oxide signaling depends on biotin in Jurkat human lymphoma cells. In: Journal of Nutrition. 2009 ; Vol. 139, No. 3. pp. 429-433.
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