nifV is contiguous to nifHDK in Frankia strain FaC1

Bo S.I. Oh, Paul Twigg, Jeong Soo Hong, Beth C. Mullin, Chung Sun An

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The organization of genes with the capacity to code for four proteins involved in nitrogen fixation in Frankia strain FaC1 was determined by restriction fragment mapping and nucleotide sequence analysis. Analysis of the 44-kb genomic cosmid clone pFAH1, isolated from a cosmid library made from Frankia strain FaC1, resulted in the identification of a 7.2-kb PstI fragment to which Klebsiella nifH, nifD and nifK probes hybridized. This nif-hybridizing fragment was subcloned and analyzed by restriction fragment mapping. Further subcloning of the 7.2-kb fragment and subsequent sequence analysis of approximately 6.8 kb revealed the presence of six open reading frames (ORFs). Four of these ORFs have the potential to code for nifV-, nifH-, nifD- and nifK-like gene products and the two others are unidentified ORFs. The organization of the structural genes for nitrogenase is the same in this Frankia strain as it is in most other nitrogen-fixing prokaryotes, but the positioning of the nifV-like gene relative to the nifHDK cluster differs. A consensus nif-promoter-like sequence, found 5' to nifH, was not detected upstream of the nifV-like gene. Nine copies of a 7-bp direct repeat were found 5' to ORFA.

Original languageEnglish (US)
Pages (from-to)707-713
Number of pages7
JournalPhysiologia Plantarum
Volume99
Issue number4
DOIs
StatePublished - Apr 1 1997

Fingerprint

Frankia
Open Reading Frames
open reading frames
Restriction Mapping
Cosmids
Genes
genes
sequence analysis
Sequence Analysis
Klebsiella
structural genes
nitrogenase
Nitrogenase
prokaryotic cells
Nitrogen Fixation
nitrogen fixation
Nucleic Acid Repetitive Sequences
promoter regions
clones
genomics

Keywords

  • Directly repeated sequence
  • Frankia strain FaC1
  • homocitrate synthase
  • nif-organization
  • nifD
  • nifH
  • nifK
  • nifV

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

nifV is contiguous to nifHDK in Frankia strain FaC1. / Oh, Bo S.I.; Twigg, Paul; Hong, Jeong Soo; Mullin, Beth C.; An, Chung Sun.

In: Physiologia Plantarum, Vol. 99, No. 4, 01.04.1997, p. 707-713.

Research output: Contribution to journalArticle

Oh, Bo S.I. ; Twigg, Paul ; Hong, Jeong Soo ; Mullin, Beth C. ; An, Chung Sun. / nifV is contiguous to nifHDK in Frankia strain FaC1. In: Physiologia Plantarum. 1997 ; Vol. 99, No. 4. pp. 707-713.
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