NF-Y binds to the CCAAT box motif of the FGF-4 gene and promotes FGF-4 expression in embryonal carcinoma cells

Kimberly A. Lamb, Lance R. Johnson, A Angie Rizzino

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

FGF-4 appears to be the first fibroblast growth factor (FGF) expressed during embryo-genesis, and its expression is critical for early mammalian development. FGF-4 is expressed in the embryonic cell lines, F9, D3, and NT2/D1; but its expression in these cells is repressed upon differentiation. Transcription of the FGF-4 gene in embryonic cells is regulated by an enhancer in the third exon and by a positive regulatory region upstream of the transcription start site. A CCAAT box motif within the positive regulatory region has been shown to support FGF-4 expression, but the factor that binds to this site in vivo has not been identified. In this report, we demonstrate that the transcription factor complex NF-Y binds to the FGF-4 CCAAT box motif when nuclear extracts from each of the embryonic cell lines and their differentiated cells were examined by gel mobility shift analyses. Importantly, we demonstrate that expression of a dominant-negative NF-YA mutant protein reduces the expression of FGF-4 promoter/reporter gene constructs in F9 EC cells. Hence, we provide strong evidence that the transcription factor NF-Y is involved in the expression of the FGF-4 gene.

Original languageEnglish (US)
Pages (from-to)301-309
Number of pages9
JournalMolecular Reproduction and Development
Volume48
Issue number3
DOIs
StatePublished - Nov 1 1997

Fingerprint

Fibroblast Growth Factor 4
Embryonal Carcinoma Stem Cells
Genes
Nucleic Acid Regulatory Sequences
Transcription Factors
Cell Line
Fibroblast Growth Factors
Transcription Initiation Site
Electrophoretic Mobility Shift Assay
Mutant Proteins
Reporter Genes
Exons
Embryonic Structures

Keywords

  • Cis-regulatory element
  • Embryonal carcinoma
  • Embryonic stem
  • Fibroblast growth factor
  • Gel mobility shift analysis

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

NF-Y binds to the CCAAT box motif of the FGF-4 gene and promotes FGF-4 expression in embryonal carcinoma cells. / Lamb, Kimberly A.; Johnson, Lance R.; Rizzino, A Angie.

In: Molecular Reproduction and Development, Vol. 48, No. 3, 01.11.1997, p. 301-309.

Research output: Contribution to journalArticle

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