New restriction endonuclease CviRI cleaves DNA at TG/CA sequences

Aiwen Jin, Yanping Zhang, Yuannan Xia, Elbert Traylor, Michael Nelson, James L. van Ettenl

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5′-TGCA-3′ and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5′-TGCA-3′ sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.

Original languageEnglish (US)
Pages (from-to)3928-3929
Number of pages2
JournalNucleic acids research
Volume22
Issue number19
DOIs
StatePublished - Sep 25 1994

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Type II Site Specific Deoxyribonucleases
Chlorella
Inborn Genetic Diseases
Cytosine
DNA Restriction Enzymes
Medical Genetics
Adenine
Methylation
Alleles
Viruses
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

New restriction endonuclease CviRI cleaves DNA at TG/CA sequences. / Jin, Aiwen; Zhang, Yanping; Xia, Yuannan; Traylor, Elbert; Nelson, Michael; van Ettenl, James L.

In: Nucleic acids research, Vol. 22, No. 19, 25.09.1994, p. 3928-3929.

Research output: Contribution to journalArticle

Jin, Aiwen ; Zhang, Yanping ; Xia, Yuannan ; Traylor, Elbert ; Nelson, Michael ; van Ettenl, James L. / New restriction endonuclease CviRI cleaves DNA at TG/CA sequences. In: Nucleic acids research. 1994 ; Vol. 22, No. 19. pp. 3928-3929.
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