New methods for the collection and preservation of spirorchiid trematodes and polystomatid monogeneans from turtles

Scott D. Snyder, Richard E. Clopton

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

New techniques and protocols for the field collection and preservation of spirorchiid trematodes and polystomatid monogeneans are delineated for inclusion in existing postmortem examination protocols for the parasites of turtles. Spirorchiid trematodes are collected in body and organ washes of citrated saline solution concentrated by sedimentation in a separately funnel. Polystomatid monogeneans are collected by precise dissection and examination of the urinary and accessory bladders, nasal and oral cavities, and the conjunctival sacs and nictitating membranes of the eye. Fixation by distilled water osmotony or heat followed by preservation and storage in 95% undenatured ethanol are recommended to produce specimens for subsequent morphological and molecular analysis.

Original languageEnglish (US)
Pages (from-to)102-107
Number of pages6
JournalComparative Parasitology
Volume72
Issue number1
DOIs
StatePublished - Jan 2005

Fingerprint

Nictitating Membrane
Lacrimal Apparatus
Turtles
Nasal Cavity
Trematoda
turtle
Sodium Chloride
turtles
Mouth
Dissection
Autopsy
Parasites
Urinary Bladder
Ethanol
Hot Temperature
lacrimal apparatus
nasal cavity
Water
molecular analysis
dissection

Keywords

  • Blood fluke
  • Chelonia
  • Collecting techniques
  • Digenea
  • Eye
  • Monogenea
  • Parasite survey
  • Platyhelminthes
  • Polystomatidae
  • Spirorchiidae
  • Trematoda
  • Turtle

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

New methods for the collection and preservation of spirorchiid trematodes and polystomatid monogeneans from turtles. / Snyder, Scott D.; Clopton, Richard E.

In: Comparative Parasitology, Vol. 72, No. 1, 01.2005, p. 102-107.

Research output: Contribution to journalArticle

@article{6e794d56da104e319cae959320cf35fa,
title = "New methods for the collection and preservation of spirorchiid trematodes and polystomatid monogeneans from turtles",
abstract = "New techniques and protocols for the field collection and preservation of spirorchiid trematodes and polystomatid monogeneans are delineated for inclusion in existing postmortem examination protocols for the parasites of turtles. Spirorchiid trematodes are collected in body and organ washes of citrated saline solution concentrated by sedimentation in a separately funnel. Polystomatid monogeneans are collected by precise dissection and examination of the urinary and accessory bladders, nasal and oral cavities, and the conjunctival sacs and nictitating membranes of the eye. Fixation by distilled water osmotony or heat followed by preservation and storage in 95{\%} undenatured ethanol are recommended to produce specimens for subsequent morphological and molecular analysis.",
keywords = "Blood fluke, Chelonia, Collecting techniques, Digenea, Eye, Monogenea, Parasite survey, Platyhelminthes, Polystomatidae, Spirorchiidae, Trematoda, Turtle",
author = "Snyder, {Scott D.} and Clopton, {Richard E.}",
year = "2005",
month = "1",
doi = "10.1654/4155",
language = "English (US)",
volume = "72",
pages = "102--107",
journal = "Comparative Parasitology",
issn = "1525-2647",
publisher = "Helminthological Society of Washington",
number = "1",

}

TY - JOUR

T1 - New methods for the collection and preservation of spirorchiid trematodes and polystomatid monogeneans from turtles

AU - Snyder, Scott D.

AU - Clopton, Richard E.

PY - 2005/1

Y1 - 2005/1

N2 - New techniques and protocols for the field collection and preservation of spirorchiid trematodes and polystomatid monogeneans are delineated for inclusion in existing postmortem examination protocols for the parasites of turtles. Spirorchiid trematodes are collected in body and organ washes of citrated saline solution concentrated by sedimentation in a separately funnel. Polystomatid monogeneans are collected by precise dissection and examination of the urinary and accessory bladders, nasal and oral cavities, and the conjunctival sacs and nictitating membranes of the eye. Fixation by distilled water osmotony or heat followed by preservation and storage in 95% undenatured ethanol are recommended to produce specimens for subsequent morphological and molecular analysis.

AB - New techniques and protocols for the field collection and preservation of spirorchiid trematodes and polystomatid monogeneans are delineated for inclusion in existing postmortem examination protocols for the parasites of turtles. Spirorchiid trematodes are collected in body and organ washes of citrated saline solution concentrated by sedimentation in a separately funnel. Polystomatid monogeneans are collected by precise dissection and examination of the urinary and accessory bladders, nasal and oral cavities, and the conjunctival sacs and nictitating membranes of the eye. Fixation by distilled water osmotony or heat followed by preservation and storage in 95% undenatured ethanol are recommended to produce specimens for subsequent morphological and molecular analysis.

KW - Blood fluke

KW - Chelonia

KW - Collecting techniques

KW - Digenea

KW - Eye

KW - Monogenea

KW - Parasite survey

KW - Platyhelminthes

KW - Polystomatidae

KW - Spirorchiidae

KW - Trematoda

KW - Turtle

UR - http://www.scopus.com/inward/record.url?scp=13644262192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13644262192&partnerID=8YFLogxK

U2 - 10.1654/4155

DO - 10.1654/4155

M3 - Article

AN - SCOPUS:13644262192

VL - 72

SP - 102

EP - 107

JO - Comparative Parasitology

JF - Comparative Parasitology

SN - 1525-2647

IS - 1

ER -