New alleles in calpastatin gene are associated with meat quality traits in pigs

Daniel C Ciobanu, J. W.M. Bastiaansen, S. M. Lonergan, H. Thomsen, J. C.M. Dekkers, G. S. Plastow, M. F. Rothschild

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Suggestive QTL affecting raw firmness scores and average Instron force, tenderness, juiciness, and chewiness on cooked meat were mapped to pig chromosome 2 using a three-generation intercross between Berkshire and Yorkshire pigs. Based on its function and location, the calpastatin (CAST) gene was considered to be a good candidate for the observed effects. Several missense and silent mutations were identified in CAST and haplotypes covering most of the coding region were constructed and used for association analyses with meat quality traits. Results demonstrated that one CAST haplotype was significantly associated with lower Instron force and cooking loss and higher juiciness and, therefore, this haplotype is associated with higher eating quality. Some of the sequence variation identified may be associated with differences in phosphorylation of CAST by adenosine cyclic 3′, 5′-monophosphate- dependent protein kinase and may in turn explain the meat quality phenotypic differences. The beneficial haplotype was present in all the commercial breeds tested and may provide significant improvements for the pig industry and consumers because it can be used in marker-assisted selection to produce naturally tender and juicy pork without additional processing steps.

Original languageEnglish (US)
Pages (from-to)2829-2839
Number of pages11
JournalJournal of animal science
Volume82
Issue number10
StatePublished - Oct 1 2004

Fingerprint

calpastatin
Meat
meat quality
Haplotypes
haplotypes
Swine
Alleles
alleles
swine
juiciness
Genes
genes
Berkshire (swine breed)
Chromosomes, Human, Pair 2
chewiness
Yorkshire (swine breed)
Cooking
cyclic AMP
Missense Mutation
cooking quality

Keywords

  • Calpastatin
  • Haplotype
  • Meat Quality
  • Pig
  • Tenderness

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Ciobanu, D. C., Bastiaansen, J. W. M., Lonergan, S. M., Thomsen, H., Dekkers, J. C. M., Plastow, G. S., & Rothschild, M. F. (2004). New alleles in calpastatin gene are associated with meat quality traits in pigs. Journal of animal science, 82(10), 2829-2839.

New alleles in calpastatin gene are associated with meat quality traits in pigs. / Ciobanu, Daniel C; Bastiaansen, J. W.M.; Lonergan, S. M.; Thomsen, H.; Dekkers, J. C.M.; Plastow, G. S.; Rothschild, M. F.

In: Journal of animal science, Vol. 82, No. 10, 01.10.2004, p. 2829-2839.

Research output: Contribution to journalArticle

Ciobanu, DC, Bastiaansen, JWM, Lonergan, SM, Thomsen, H, Dekkers, JCM, Plastow, GS & Rothschild, MF 2004, 'New alleles in calpastatin gene are associated with meat quality traits in pigs', Journal of animal science, vol. 82, no. 10, pp. 2829-2839.
Ciobanu DC, Bastiaansen JWM, Lonergan SM, Thomsen H, Dekkers JCM, Plastow GS et al. New alleles in calpastatin gene are associated with meat quality traits in pigs. Journal of animal science. 2004 Oct 1;82(10):2829-2839.
Ciobanu, Daniel C ; Bastiaansen, J. W.M. ; Lonergan, S. M. ; Thomsen, H. ; Dekkers, J. C.M. ; Plastow, G. S. ; Rothschild, M. F. / New alleles in calpastatin gene are associated with meat quality traits in pigs. In: Journal of animal science. 2004 ; Vol. 82, No. 10. pp. 2829-2839.
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