Neutrophilic respiratory tract inflammation and peripheral blood neutrophilia after grain sorghum dust extract challenge

Susanna G Von Essen, D. P. O'Neill, S. McGranaghan, S. A. Olenchock, S. I. Rennard

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Study objective: To determine if inhalation of grain sorghum dust in the laboratory would cause neutrophilic upper and lower respiratory tract inflammation in human volunteers, as well as systemic signs of illness. Design: Prospective. Setting: University of Nebraska Medical Center. Participants: Thirty normal volunteers. Interventions: Inhalation challenge with 20 mL of a nebulized solution of filter-sterilized grain sorghum dust extract (GSDE). One group received prednisone, 20 mg for 2 days, prior to the challenge. Measurements and results: Bronchoscopy with bronchoalveolar lavage (BAL) was performed 24 h after challenge, with samples collected as bronchial and alveolar fractions. Findings included visible signs of airways inflammation, quantified as the bronchitis index. The percentage of bronchial neutrophils was significantly increased in those challenged with GSDE vs the control solution, Hanks' balanced salt solution (40.3±4.5% vs 14.3±5.1%, p≤.01). Similar findings were seen in the alveolar fraction. Pretreatment with corticosteroids did not prevent the rise in neutrophils recovered by BAL. Peripheral blood neutrophils were also increased in volunteers challenged with the grain dust extract. To explain the increase in peripheral blood neutrophil counts, the capacity of the peripheral blood neutrophils to migrate in chemotaxis experiments was examined. The results demonstrate an increase in peripheral blood neutrophils and an increase in chemotactic responsiveness. Conclusions: Inhalation challenge with a grain dust extract causes respiratory tract inflammation and a peripheral blood neutrophilia. One reason for this may be an increase in activated peripheral blood neutrophils.

Original languageEnglish (US)
Pages (from-to)1425-1433
Number of pages9
JournalChest
Volume108
Issue number5
DOIs
StatePublished - Jan 1 1995

Fingerprint

Sorghum
Dust
Respiratory System
Neutrophils
Inflammation
Inhalation
Bronchoalveolar Lavage
Volunteers
Bronchitis
Bronchoscopy
Chemotaxis
Prednisone
Healthy Volunteers
Adrenal Cortex Hormones

Keywords

  • bronchitis
  • bronchoalveolar lavage
  • grain dust
  • inhalation challenge
  • nasal lavage
  • neutrophils

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Neutrophilic respiratory tract inflammation and peripheral blood neutrophilia after grain sorghum dust extract challenge. / Von Essen, Susanna G; O'Neill, D. P.; McGranaghan, S.; Olenchock, S. A.; Rennard, S. I.

In: Chest, Vol. 108, No. 5, 01.01.1995, p. 1425-1433.

Research output: Contribution to journalArticle

Von Essen, Susanna G ; O'Neill, D. P. ; McGranaghan, S. ; Olenchock, S. A. ; Rennard, S. I. / Neutrophilic respiratory tract inflammation and peripheral blood neutrophilia after grain sorghum dust extract challenge. In: Chest. 1995 ; Vol. 108, No. 5. pp. 1425-1433.
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abstract = "Study objective: To determine if inhalation of grain sorghum dust in the laboratory would cause neutrophilic upper and lower respiratory tract inflammation in human volunteers, as well as systemic signs of illness. Design: Prospective. Setting: University of Nebraska Medical Center. Participants: Thirty normal volunteers. Interventions: Inhalation challenge with 20 mL of a nebulized solution of filter-sterilized grain sorghum dust extract (GSDE). One group received prednisone, 20 mg for 2 days, prior to the challenge. Measurements and results: Bronchoscopy with bronchoalveolar lavage (BAL) was performed 24 h after challenge, with samples collected as bronchial and alveolar fractions. Findings included visible signs of airways inflammation, quantified as the bronchitis index. The percentage of bronchial neutrophils was significantly increased in those challenged with GSDE vs the control solution, Hanks' balanced salt solution (40.3±4.5{\%} vs 14.3±5.1{\%}, p≤.01). Similar findings were seen in the alveolar fraction. Pretreatment with corticosteroids did not prevent the rise in neutrophils recovered by BAL. Peripheral blood neutrophils were also increased in volunteers challenged with the grain dust extract. To explain the increase in peripheral blood neutrophil counts, the capacity of the peripheral blood neutrophils to migrate in chemotaxis experiments was examined. The results demonstrate an increase in peripheral blood neutrophils and an increase in chemotactic responsiveness. Conclusions: Inhalation challenge with a grain dust extract causes respiratory tract inflammation and a peripheral blood neutrophilia. One reason for this may be an increase in activated peripheral blood neutrophils.",
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