Neutrophil proteome shifts over the myocardial infarction time continuum

Michael J. Daseke, Fritz M. Valerio, William J. Kalusche, Yonggang Ma, Kristine Y. DeLeon-Pennell, Merry L Lindsey

Research output: Contribution to journalArticle

Abstract

In response to myocardial infarction (MI), neutrophils (PMNs) are early responders that initiate the inflammatory reaction. Because macrophages and fibroblasts show polarization states after MI, we hypothesized PMNs also undergo phenotypic changes over the MI time course. The objective of the current study was to map the continuum of polarization phenotypes in cardiac neutrophils over the first week of MI. C57BL/6J male mice (3–6 months old) underwent permanent coronary artery ligation to induce MI, and PMNs were isolated from the infarct region at days 1, 3, 5, and 7 after MI. Day 0 served as a no MI negative control. Aptamer proteomics was performed on biological replicates (n = 10–12) for each time point. Day (D)1 MI neutrophils had a high degranulation profile with increased matrix metalloproteinase (MMP) activity. D3 MI neutrophil profiles showed upregulation of apoptosis and induction of extracellular matrix (ECM) organization. D5 MI neutrophils further increased their ECM reorganization profile. D7 MI neutrophils had a reparative signature that included expression of fibronectin, galectin-3, and fibrinogen to contribute to scar formation by stimulating ECM reorganization. Of note, fibronectin was a key modulator of degranulation, as it amplified MMP-9 release in the presence of an inflammatory stimulus. Our results indicate that neutrophils selectively degranulate over the MI time course, reflective of both their intrinsic protein profiles as well as the ECM environment in which they reside. MMPs, cathepsins, and ECM proteins were prominent neutrophil degranulation indicators.

Original languageEnglish (US)
Article number37
JournalBasic research in cardiology
Volume114
Issue number5
DOIs
StatePublished - Sep 1 2019

Fingerprint

Proteome
Neutrophils
Myocardial Infarction
Extracellular Matrix
Matrix Metalloproteinases
Fibronectins
Galectin 3
Cathepsins
Extracellular Matrix Proteins
Matrix Metalloproteinase 9
Proteomics
Fibrinogen
Cicatrix
Ligation
Coronary Vessels
Up-Regulation
Fibroblasts
Macrophages
Apoptosis
Phenotype

Keywords

  • Aptamer
  • Cell polarization
  • LV remodeling
  • Myocardial infarction
  • Neutrophil
  • Proteomics

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Neutrophil proteome shifts over the myocardial infarction time continuum. / Daseke, Michael J.; Valerio, Fritz M.; Kalusche, William J.; Ma, Yonggang; DeLeon-Pennell, Kristine Y.; Lindsey, Merry L.

In: Basic research in cardiology, Vol. 114, No. 5, 37, 01.09.2019.

Research output: Contribution to journalArticle

Daseke, Michael J. ; Valerio, Fritz M. ; Kalusche, William J. ; Ma, Yonggang ; DeLeon-Pennell, Kristine Y. ; Lindsey, Merry L. / Neutrophil proteome shifts over the myocardial infarction time continuum. In: Basic research in cardiology. 2019 ; Vol. 114, No. 5.
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