Neuropeptide Y inhibition of nicotinic receptor-mediated chromaffin cell secretion

T. D. Hexum, Jialin C Zheng, J. Zhu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Neuropeptide Y (NPY), a widely distributed peptide with varied activities, inhibits nicotinic receptor-induced [3H]norepinephrine ([3H]NE) secretion from bovine chromaffin cells. The secretion produced by membrane depolarization with high KCl concentrations or veratridine is not inhibited. Fragments of NPY, such as NPY18-36, are potent inhibitors of [3H]NE secretion, whereas [Leu31,Pro34]-NPY and peptide YY have no effect. The response to NPY18-36 is not sensitive to pertussis toxin pretreatment of chromaffin cells. NPY fragments also inhibit nicotinic receptor-induced 45Ca++ influx but not that induced by KCl or veratridine. The rank orders of potency for inhibition of [3H]NE secretion and 45Ca++ influx are the same: NPY18-36 ≥ NPY26-36 > NPY13-36. NPY and NPY(free acid) are weak inhibitors of secretion but not 45Ca++ influx. Moreover, the IC50s for NPY18-36 inhibition of [3H]NE secretion and 45Ca++ influx are comparable, 1.4 x 10-6 M and 0.9 x 10-6 M, respectively. Regression analysis produced a correlation coefficient of 0.9842 (P < .0001). It was concluded that NPY inhibits [3H]NE secretion by a modification of the nicotinic receptor-mediated increase in Ca++ influx. The characterization of the response suggests that the NPY effect is mediated by a previously undefined NPY receptor subtype that was designated Y4.

Original languageEnglish (US)
Pages (from-to)61-66
Number of pages6
JournalJournal of Pharmacology and Experimental Therapeutics
Volume271
Issue number1
StatePublished - Jan 1 1994

Fingerprint

Chromaffin Cells
Neuropeptide Y
Nicotinic Receptors
Veratridine
Neuropeptide Y Receptors
Peptide YY
Pertussis Toxin
Norepinephrine
Regression Analysis
Peptides
Acids
Membranes

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Neuropeptide Y inhibition of nicotinic receptor-mediated chromaffin cell secretion. / Hexum, T. D.; Zheng, Jialin C; Zhu, J.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 271, No. 1, 01.01.1994, p. 61-66.

Research output: Contribution to journalArticle

@article{ecf6af33494b4a938842ab721d22c04e,
title = "Neuropeptide Y inhibition of nicotinic receptor-mediated chromaffin cell secretion",
abstract = "Neuropeptide Y (NPY), a widely distributed peptide with varied activities, inhibits nicotinic receptor-induced [3H]norepinephrine ([3H]NE) secretion from bovine chromaffin cells. The secretion produced by membrane depolarization with high KCl concentrations or veratridine is not inhibited. Fragments of NPY, such as NPY18-36, are potent inhibitors of [3H]NE secretion, whereas [Leu31,Pro34]-NPY and peptide YY have no effect. The response to NPY18-36 is not sensitive to pertussis toxin pretreatment of chromaffin cells. NPY fragments also inhibit nicotinic receptor-induced 45Ca++ influx but not that induced by KCl or veratridine. The rank orders of potency for inhibition of [3H]NE secretion and 45Ca++ influx are the same: NPY18-36 ≥ NPY26-36 > NPY13-36. NPY and NPY(free acid) are weak inhibitors of secretion but not 45Ca++ influx. Moreover, the IC50s for NPY18-36 inhibition of [3H]NE secretion and 45Ca++ influx are comparable, 1.4 x 10-6 M and 0.9 x 10-6 M, respectively. Regression analysis produced a correlation coefficient of 0.9842 (P < .0001). It was concluded that NPY inhibits [3H]NE secretion by a modification of the nicotinic receptor-mediated increase in Ca++ influx. The characterization of the response suggests that the NPY effect is mediated by a previously undefined NPY receptor subtype that was designated Y4.",
author = "Hexum, {T. D.} and Zheng, {Jialin C} and J. Zhu",
year = "1994",
month = "1",
day = "1",
language = "English (US)",
volume = "271",
pages = "61--66",
journal = "Journal of Pharmacology and Experimental Therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "1",

}

TY - JOUR

T1 - Neuropeptide Y inhibition of nicotinic receptor-mediated chromaffin cell secretion

AU - Hexum, T. D.

AU - Zheng, Jialin C

AU - Zhu, J.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Neuropeptide Y (NPY), a widely distributed peptide with varied activities, inhibits nicotinic receptor-induced [3H]norepinephrine ([3H]NE) secretion from bovine chromaffin cells. The secretion produced by membrane depolarization with high KCl concentrations or veratridine is not inhibited. Fragments of NPY, such as NPY18-36, are potent inhibitors of [3H]NE secretion, whereas [Leu31,Pro34]-NPY and peptide YY have no effect. The response to NPY18-36 is not sensitive to pertussis toxin pretreatment of chromaffin cells. NPY fragments also inhibit nicotinic receptor-induced 45Ca++ influx but not that induced by KCl or veratridine. The rank orders of potency for inhibition of [3H]NE secretion and 45Ca++ influx are the same: NPY18-36 ≥ NPY26-36 > NPY13-36. NPY and NPY(free acid) are weak inhibitors of secretion but not 45Ca++ influx. Moreover, the IC50s for NPY18-36 inhibition of [3H]NE secretion and 45Ca++ influx are comparable, 1.4 x 10-6 M and 0.9 x 10-6 M, respectively. Regression analysis produced a correlation coefficient of 0.9842 (P < .0001). It was concluded that NPY inhibits [3H]NE secretion by a modification of the nicotinic receptor-mediated increase in Ca++ influx. The characterization of the response suggests that the NPY effect is mediated by a previously undefined NPY receptor subtype that was designated Y4.

AB - Neuropeptide Y (NPY), a widely distributed peptide with varied activities, inhibits nicotinic receptor-induced [3H]norepinephrine ([3H]NE) secretion from bovine chromaffin cells. The secretion produced by membrane depolarization with high KCl concentrations or veratridine is not inhibited. Fragments of NPY, such as NPY18-36, are potent inhibitors of [3H]NE secretion, whereas [Leu31,Pro34]-NPY and peptide YY have no effect. The response to NPY18-36 is not sensitive to pertussis toxin pretreatment of chromaffin cells. NPY fragments also inhibit nicotinic receptor-induced 45Ca++ influx but not that induced by KCl or veratridine. The rank orders of potency for inhibition of [3H]NE secretion and 45Ca++ influx are the same: NPY18-36 ≥ NPY26-36 > NPY13-36. NPY and NPY(free acid) are weak inhibitors of secretion but not 45Ca++ influx. Moreover, the IC50s for NPY18-36 inhibition of [3H]NE secretion and 45Ca++ influx are comparable, 1.4 x 10-6 M and 0.9 x 10-6 M, respectively. Regression analysis produced a correlation coefficient of 0.9842 (P < .0001). It was concluded that NPY inhibits [3H]NE secretion by a modification of the nicotinic receptor-mediated increase in Ca++ influx. The characterization of the response suggests that the NPY effect is mediated by a previously undefined NPY receptor subtype that was designated Y4.

UR - http://www.scopus.com/inward/record.url?scp=0027938085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027938085&partnerID=8YFLogxK

M3 - Article

C2 - 7965758

AN - SCOPUS:0027938085

VL - 271

SP - 61

EP - 66

JO - Journal of Pharmacology and Experimental Therapeutics

JF - Journal of Pharmacology and Experimental Therapeutics

SN - 0022-3565

IS - 1

ER -