Neuropeptide y enhances ATP-induced formation of inositol phosphates in chromaffin cells

Jialin C Zheng, Peijin Zhang, Myron Lee Toews, Terry D. Hexum

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Bovine chromaffin cells contain high affinity NPY binding sites coupled through a pertussis toxin-sensitive G protein to inhibition of cAMP accumulation. NPY alone does not alter [3H]inositol phosphate formation from [3H]phosphoinositides in these cells. Increasing NPY concentrations, in the presence of ATP (300 μM), produced a dose-dependent enhancement in [3H]inositol phosphate formation, EC50 = 3.2 nM. Inclusion of the selective NPY-Y1 receptor antagonist BW1229 (1 μM) produced a marked decrease in NPY potency (EC50 = 3.3 μM). The Y1 receptor agonist, [Leu31, pro34]NPY, was equally effective with NPY, whereas NPY1836, a Y2 receptor agonist, was much less effective. Inclusion of NPY with ATP also produced an enhancement in the release of intracellular Ca2+. The ability of NPY to enhance both [3H]inositol phosphate formation and the release of intracellular Ca2+ was pertussis toxin-insensitive. NPY action on bovine chromaffin cell receptor(s) appears to facilitated by different G proteins: one which can inhibit cAMP accumulation via a pertussis toxin-sensitive process and another which can enhance ATP activation of the inositol phosphate signaling pathway by a pertussis toxin-insensitive process.

Original languageEnglish (US)
Pages (from-to)287-290
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number1
DOIs
StatePublished - Oct 9 1997

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Chromaffin Cells
Inositol Phosphates
Pertussis Toxin
Neuropeptides
Adenosine Triphosphate
GTP-Binding Proteins
Phosphatidylinositols
Chemical activation
Binding Sites

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Neuropeptide y enhances ATP-induced formation of inositol phosphates in chromaffin cells. / Zheng, Jialin C; Zhang, Peijin; Toews, Myron Lee; Hexum, Terry D.

In: Biochemical and Biophysical Research Communications, Vol. 239, No. 1, 09.10.1997, p. 287-290.

Research output: Contribution to journalArticle

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