Neuropeptide γ‐(l‐9)‐Peptide: A Major Product of the Posttranslational Processing of γ‐Preprotachykinin in Rat Tissues

Yunxia Wang, Charles S. Bockman, Sandor Lovas, Peter W. Abel, Richard F. Murphy, J. Michael Conlon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Abstract: γ‐Preprotachykinin mRNA is the most abundant tachykinin mRNA in rat tissues, but the pathway of posttranslational processing of its translation product is unknown. An antiserum was raised against the synthetic peptide Asp‐Ala‐Gly‐His‐Gly‐Gln‐lle‐Ser‐His [neuropeptide γ‐(1‐9)‐peptide, equivalent to γ‐preprotachykinin‐(72‐80)‐peptide], that showed <1% reactivity with intact neuropeptide γ and other tachykinins. Neuropeptide γ‐(1‐9)‐peptide was detected by radioimmunoassay in relatively high concentrations in extracts of regions of rat brain and gastrointestinal tract. These concentrations correlated with (r = 0.99), but were significantly (p < 0.05) less than, the concentrations of neurokinin A‐like immunoreactivity. The neuropeptide γ‐(1‐9)‐like immunoreactivity in an extract of rat brain was eluted from a reverse‐phase HPLC column in a single fraction with the same retention time as synthetic neuropeptide γ‐(1 ‐9)‐peptide. The synthetic peptide did not contract or relax isolated rat trachea, superior mesenteric artery, stomach fundus, or ileum, and the peptide did not affect the ability of neuropeptide 7 to contract the rat fundus. It is concluded that, in rat tissues, Lys70‐Arg71 in 7‐preprotachykinin is a major site of posttranslational processing, but the resulting product, neuropeptide γ‐(1‐9)‐peptide, is neither an agonist nor an antagonist at the neurokinin‐2 (NK‐2) receptor.

Original languageEnglish (US)
Pages (from-to)1231-1235
Number of pages5
JournalJournal of Neurochemistry
Volume61
Issue number4
DOIs
StatePublished - Oct 1993

Fingerprint

Neuropeptides
Rats
Tissue
Peptides
Processing
Tachykinins
Brain
Messenger RNA
Superior Mesenteric Artery
peptide A
preprotachykinin
Trachea
Ileum
Radioimmunoassay
Gastrointestinal Tract
Immune Sera
Stomach
High Pressure Liquid Chromatography

Keywords

  • Neurokinin A
  • Neuropeptide γ
  • Posttranslational processing
  • Preprotachykinin
  • Rat brain

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Neuropeptide γ‐(l‐9)‐Peptide : A Major Product of the Posttranslational Processing of γ‐Preprotachykinin in Rat Tissues. / Wang, Yunxia; Bockman, Charles S.; Lovas, Sandor; Abel, Peter W.; Murphy, Richard F.; Conlon, J. Michael.

In: Journal of Neurochemistry, Vol. 61, No. 4, 10.1993, p. 1231-1235.

Research output: Contribution to journalArticle

Wang, Yunxia ; Bockman, Charles S. ; Lovas, Sandor ; Abel, Peter W. ; Murphy, Richard F. ; Conlon, J. Michael. / Neuropeptide γ‐(l‐9)‐Peptide : A Major Product of the Posttranslational Processing of γ‐Preprotachykinin in Rat Tissues. In: Journal of Neurochemistry. 1993 ; Vol. 61, No. 4. pp. 1231-1235.
@article{cc9cb659ad2c4c3a83d5a52a95c24f83,
title = "Neuropeptide γ‐(l‐9)‐Peptide: A Major Product of the Posttranslational Processing of γ‐Preprotachykinin in Rat Tissues",
abstract = "Abstract: γ‐Preprotachykinin mRNA is the most abundant tachykinin mRNA in rat tissues, but the pathway of posttranslational processing of its translation product is unknown. An antiserum was raised against the synthetic peptide Asp‐Ala‐Gly‐His‐Gly‐Gln‐lle‐Ser‐His [neuropeptide γ‐(1‐9)‐peptide, equivalent to γ‐preprotachykinin‐(72‐80)‐peptide], that showed <1{\%} reactivity with intact neuropeptide γ and other tachykinins. Neuropeptide γ‐(1‐9)‐peptide was detected by radioimmunoassay in relatively high concentrations in extracts of regions of rat brain and gastrointestinal tract. These concentrations correlated with (r = 0.99), but were significantly (p < 0.05) less than, the concentrations of neurokinin A‐like immunoreactivity. The neuropeptide γ‐(1‐9)‐like immunoreactivity in an extract of rat brain was eluted from a reverse‐phase HPLC column in a single fraction with the same retention time as synthetic neuropeptide γ‐(1 ‐9)‐peptide. The synthetic peptide did not contract or relax isolated rat trachea, superior mesenteric artery, stomach fundus, or ileum, and the peptide did not affect the ability of neuropeptide 7 to contract the rat fundus. It is concluded that, in rat tissues, Lys70‐Arg71 in 7‐preprotachykinin is a major site of posttranslational processing, but the resulting product, neuropeptide γ‐(1‐9)‐peptide, is neither an agonist nor an antagonist at the neurokinin‐2 (NK‐2) receptor.",
keywords = "Neurokinin A, Neuropeptide γ, Posttranslational processing, Preprotachykinin, Rat brain",
author = "Yunxia Wang and Bockman, {Charles S.} and Sandor Lovas and Abel, {Peter W.} and Murphy, {Richard F.} and Conlon, {J. Michael}",
year = "1993",
month = "10",
doi = "10.1111/j.1471-4159.1993.tb13613.x",
language = "English (US)",
volume = "61",
pages = "1231--1235",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Neuropeptide γ‐(l‐9)‐Peptide

T2 - A Major Product of the Posttranslational Processing of γ‐Preprotachykinin in Rat Tissues

AU - Wang, Yunxia

AU - Bockman, Charles S.

AU - Lovas, Sandor

AU - Abel, Peter W.

AU - Murphy, Richard F.

AU - Conlon, J. Michael

PY - 1993/10

Y1 - 1993/10

N2 - Abstract: γ‐Preprotachykinin mRNA is the most abundant tachykinin mRNA in rat tissues, but the pathway of posttranslational processing of its translation product is unknown. An antiserum was raised against the synthetic peptide Asp‐Ala‐Gly‐His‐Gly‐Gln‐lle‐Ser‐His [neuropeptide γ‐(1‐9)‐peptide, equivalent to γ‐preprotachykinin‐(72‐80)‐peptide], that showed <1% reactivity with intact neuropeptide γ and other tachykinins. Neuropeptide γ‐(1‐9)‐peptide was detected by radioimmunoassay in relatively high concentrations in extracts of regions of rat brain and gastrointestinal tract. These concentrations correlated with (r = 0.99), but were significantly (p < 0.05) less than, the concentrations of neurokinin A‐like immunoreactivity. The neuropeptide γ‐(1‐9)‐like immunoreactivity in an extract of rat brain was eluted from a reverse‐phase HPLC column in a single fraction with the same retention time as synthetic neuropeptide γ‐(1 ‐9)‐peptide. The synthetic peptide did not contract or relax isolated rat trachea, superior mesenteric artery, stomach fundus, or ileum, and the peptide did not affect the ability of neuropeptide 7 to contract the rat fundus. It is concluded that, in rat tissues, Lys70‐Arg71 in 7‐preprotachykinin is a major site of posttranslational processing, but the resulting product, neuropeptide γ‐(1‐9)‐peptide, is neither an agonist nor an antagonist at the neurokinin‐2 (NK‐2) receptor.

AB - Abstract: γ‐Preprotachykinin mRNA is the most abundant tachykinin mRNA in rat tissues, but the pathway of posttranslational processing of its translation product is unknown. An antiserum was raised against the synthetic peptide Asp‐Ala‐Gly‐His‐Gly‐Gln‐lle‐Ser‐His [neuropeptide γ‐(1‐9)‐peptide, equivalent to γ‐preprotachykinin‐(72‐80)‐peptide], that showed <1% reactivity with intact neuropeptide γ and other tachykinins. Neuropeptide γ‐(1‐9)‐peptide was detected by radioimmunoassay in relatively high concentrations in extracts of regions of rat brain and gastrointestinal tract. These concentrations correlated with (r = 0.99), but were significantly (p < 0.05) less than, the concentrations of neurokinin A‐like immunoreactivity. The neuropeptide γ‐(1‐9)‐like immunoreactivity in an extract of rat brain was eluted from a reverse‐phase HPLC column in a single fraction with the same retention time as synthetic neuropeptide γ‐(1 ‐9)‐peptide. The synthetic peptide did not contract or relax isolated rat trachea, superior mesenteric artery, stomach fundus, or ileum, and the peptide did not affect the ability of neuropeptide 7 to contract the rat fundus. It is concluded that, in rat tissues, Lys70‐Arg71 in 7‐preprotachykinin is a major site of posttranslational processing, but the resulting product, neuropeptide γ‐(1‐9)‐peptide, is neither an agonist nor an antagonist at the neurokinin‐2 (NK‐2) receptor.

KW - Neurokinin A

KW - Neuropeptide γ

KW - Posttranslational processing

KW - Preprotachykinin

KW - Rat brain

UR - http://www.scopus.com/inward/record.url?scp=0027490895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027490895&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1993.tb13613.x

DO - 10.1111/j.1471-4159.1993.tb13613.x

M3 - Article

C2 - 8376981

AN - SCOPUS:0027490895

VL - 61

SP - 1231

EP - 1235

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 4

ER -