Neuropathy and anti‐myelin‐associated glycoprotein IgM M proteins: T cell regulation of M protein secretion in vitro

Norman Latov, Maurice Godfrey, Yoland Thomas, Eduardo Nobile‐Orazio, Linda Spatz, Judith Abraham, Gayle Perman, Lorenza Freddo, Leonard Chess

Research output: Contribution to journalArticle

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Abstract

In patients with plasma cell dyscrasia, individual clones of antibody‐producing cells proliferate abnormally and secrete monoclonal antibodies or M proteins in excess. The cause of the monoclonal proliferation of lymphocytes and M protein secretion is unknown and it is not known whether the M protein–secreting B cells are autonomous or capable of responding to regulatory T cells. We carried out experiments using lymphocytes from a patient with neuropathy and plasma cell dyscrasia whose IgM M protein bound to the myelin‐associated glycoprotein (MAG) to determine whether secretion of the M protein in vitro was responsive to T cell help or suppression. M protein secretion was measured by an enzyme‐linked immunosorbent assay system for measuring anti‐MAG IgM, and the number of M protein–secreting lymphocytes was enumerated by a reverse hemolytic plaque assay specific for the M protein idiotype. The patient's B cells were maximally stimulated by pokeweed mitogen–activated autologous OKT4+ T‐helper cells and the helper effect was inhibited by OKT8+ suppressor/cytotoxic T cells. Low levels of M protein secretion in the absence of T cells were also observed and there was partial stimulation of M protein secretion by T cells in the absence of pokeweed mitogen.

Original languageEnglish (US)
Pages (from-to)182-188
Number of pages7
JournalAnnals of Neurology
Volume18
Issue number2
DOIs
StatePublished - Aug 1985

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Immunoglobulin M
T-Lymphocytes
Proteins
Paraproteinemias
Lymphocytes
B-Lymphocytes
Phytolacca americana
Pokeweed Mitogens
Immunosorbents
In Vitro Techniques
protein M (glycoprotein)
Regulatory T-Lymphocytes
Helper-Inducer T-Lymphocytes
Glycoproteins
Clone Cells
Monoclonal Antibodies

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Neuropathy and anti‐myelin‐associated glycoprotein IgM M proteins : T cell regulation of M protein secretion in vitro. / Latov, Norman; Godfrey, Maurice; Thomas, Yoland; Nobile‐Orazio, Eduardo; Spatz, Linda; Abraham, Judith; Perman, Gayle; Freddo, Lorenza; Chess, Leonard.

In: Annals of Neurology, Vol. 18, No. 2, 08.1985, p. 182-188.

Research output: Contribution to journalArticle

Latov, N, Godfrey, M, Thomas, Y, Nobile‐Orazio, E, Spatz, L, Abraham, J, Perman, G, Freddo, L & Chess, L 1985, 'Neuropathy and anti‐myelin‐associated glycoprotein IgM M proteins: T cell regulation of M protein secretion in vitro', Annals of Neurology, vol. 18, no. 2, pp. 182-188. https://doi.org/10.1002/ana.410180204
Latov, Norman ; Godfrey, Maurice ; Thomas, Yoland ; Nobile‐Orazio, Eduardo ; Spatz, Linda ; Abraham, Judith ; Perman, Gayle ; Freddo, Lorenza ; Chess, Leonard. / Neuropathy and anti‐myelin‐associated glycoprotein IgM M proteins : T cell regulation of M protein secretion in vitro. In: Annals of Neurology. 1985 ; Vol. 18, No. 2. pp. 182-188.
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