Neuronal uptake and intracellular superoxide scavenging of a fullerene (C60)-poly(2-oxazoline)s nanoformulation

Jing Tong, Matthew C Zimmerman, Shumin Li, Xiang Yi, Robert Luxenhofer, Rainer Jordan, Alexander V. Kabanov

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Fullerene, the third allotrope of carbon, has been referred to as a "radical sponge" because of its powerful radical scavenging activities. However, the hydrophobicity and toxicity associated with fullerene limits its application as a therapeutic antioxidant. In the present study, we sought to overcome these limitations by generating water-soluble nanoformulations of fullerene (C60). Fullerene (C60) was formulated with poly(N-vinyl pyrrolidine) (PVP) or poly(2-alkyl-2-oxazoline)s (POx) homopolymer and random copolymer to form nano-complexes. These C60-polymer complexes were characterized by UV-vis spectroscopy, infrared spectroscopy (IR), dynamic light scattering (DLS), atomic force microscopy (AFM) and transmission electron microscopy (TEM). Cellular uptake and intracellular distribution of the selected formulations in catecholaminergic (CATH.a) neurons were examined by UV-vis spectroscopy, immunofluorescence and immunogold labeling. Electron paramagnetic resonance (EPR) spectroscopy was used to determine the ability of these C60-polymer complexes to scavenge superoxide. Their cytotoxicity was evaluated in three different cell lines. C60-POx and C60-PVP complexes exhibited similar physicochemical properties and antioxidant activities. C60-poly(2-ethyl-2-oxazoline) (PEtOx) complex, but not C60-PVP complex, were efficiently taken up by CATH.a neurons and attenuated the increase in intra-neuronal superoxide induced by angiotensin II (Ang II) stimulation. These results show that C60-POx complexes are non-toxic, neuronal cell permeable, superoxide scavenging antioxidants that might be promising candidates for the treatment of brain-related diseases associated with increased levels of superoxide.

Original languageEnglish (US)
Pages (from-to)3654-3665
Number of pages12
JournalBiomaterials
Volume32
Issue number14
DOIs
StatePublished - May 1 2011

Fingerprint

Scavenging
Fullerenes
Superoxides
Spectrum Analysis
Antioxidants
Ultraviolet spectroscopy
Neurons
Polymers
Atomic Force Microscopy
Electron Spin Resonance Spectroscopy
Brain Diseases
Porifera
Dynamic light scattering
Cytotoxicity
Hydrophobicity
Homopolymerization
Transmission Electron Microscopy
Hydrophobic and Hydrophilic Interactions
Angiotensin II
Labeling

Keywords

  • Antioxidant
  • Free radical
  • Fullerene (C60)
  • Neural cell
  • Polyoxazoline

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Neuronal uptake and intracellular superoxide scavenging of a fullerene (C60)-poly(2-oxazoline)s nanoformulation. / Tong, Jing; Zimmerman, Matthew C; Li, Shumin; Yi, Xiang; Luxenhofer, Robert; Jordan, Rainer; Kabanov, Alexander V.

In: Biomaterials, Vol. 32, No. 14, 01.05.2011, p. 3654-3665.

Research output: Contribution to journalArticle

Tong, Jing ; Zimmerman, Matthew C ; Li, Shumin ; Yi, Xiang ; Luxenhofer, Robert ; Jordan, Rainer ; Kabanov, Alexander V. / Neuronal uptake and intracellular superoxide scavenging of a fullerene (C60)-poly(2-oxazoline)s nanoformulation. In: Biomaterials. 2011 ; Vol. 32, No. 14. pp. 3654-3665.
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