Neuromodulatory activities of CD4+CD25+ regulatory T cells in a murine model of HIV-1-associated neurodegeneration

Jianuo Liu, Nan Gong, Xiuyan Huang, Ashley D. Reynolds, R Lee Mosley, Howard Eliot Gendelman

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

HIV-1-associated neurocognitive impairments are intrinsically linked to microglial immune activation, persistent viral infection, and inflammation. In the era of antiretroviral therapy, more subtle cognitive impairments occur without adaptive immune compromise. We posit that adaptive immunity is neuroprotective, serving in both the elimination of infected cells through CD8+ cytotoxic T cell activities and the regulation of neuroinflammatory responses of activated microglia. For the latter, little is known. Thus, we studied the neuromodulatory effects of CD4+ regulatory T cells (Treg; CD4+CD25+) or effector T cells in HIV-1-associated neurodegeneration. A newly developed HIV-1 encephalitis mouse model was used wherein murine bone marrow-derived macrophages are infected with a full-length HIV-1YU2/vesicular stomatitis viral pseudotype and injected into basal ganglia of syngeneic immunocompetent mice. Adoptive transfer of CD3-activated Treg attenuated astrogliosis and microglia inflammation with concomitant neuroprotection. Moreover, Treg-mediated anti-inflammatory activities and neuroprotection were associated with up-regulation of brain-derived neurotrophic factor and glial cell-derived neurotrophic factor expression and down-regulation of proinflammatory cytokines, oxidative stress, and viral replication. Effector T cells showed contrary effects. These results, taken together, demonstrate the importance of Treg in disease control and raise the possibility of their utility for therapeutic strategies.

Original languageEnglish (US)
Pages (from-to)3855-3865
Number of pages11
JournalJournal of Immunology
Volume182
Issue number6
DOIs
StatePublished - Mar 15 2009

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Regulatory T-Lymphocytes
HIV-1
Microglia
T-Lymphocytes
Inflammation
Vesicular Stomatitis
Adoptive Transfer
Brain-Derived Neurotrophic Factor
Nerve Growth Factors
Adaptive Immunity
Virus Diseases
Encephalitis
Basal Ganglia
Neuroglia
Oxidative Stress
Anti-Inflammatory Agents
Up-Regulation
Down-Regulation
Macrophages
HIV

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Neuromodulatory activities of CD4+CD25+ regulatory T cells in a murine model of HIV-1-associated neurodegeneration. / Liu, Jianuo; Gong, Nan; Huang, Xiuyan; Reynolds, Ashley D.; Mosley, R Lee; Gendelman, Howard Eliot.

In: Journal of Immunology, Vol. 182, No. 6, 15.03.2009, p. 3855-3865.

Research output: Contribution to journalArticle

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