Neuroinflammation alters voltage-dependent conductance in striatal astrocytes

Nikolay Karpuk, Maria Burkovetskaya, Tammy L Kielian

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Neuroinflammation has the capacity to alter normal central nervous system (CNS) homeostasis and function. The objective of the present study was to examine the effects of an inflammatory milieu on the electrophysiological properties of striatal astrocyte subpopulations with a mouse bacterial brain abscess model. Whole cell patch-clamp recordings were performed in striatal glial fibrillary acidic protein (GFAP)-green fluorescent protein (GFP)+ astrocytes neighboring abscesses at postinfection days 3 or 7 in adult mice. Cell input conductance (Gi) measurements spanning a membrane potential (Vm) surrounding resting membrane potential (RMP) revealed two prevalent astrocyte subsets. A1 and A2 astrocytes were identified by negative and positive Gi increments vs. Vm, respectively. A1 and A2 astrocytes displayed significantly different RMP, Gi, and cell membrane capacitance that were influenced by both time after bacterial exposure and astrocyte proximity to the inflammatory site. Specifically, the percentage of A1 astrocytes was decreased immediately surrounding the inflammatory lesion, whereas A2 cells were increased. These changes were particularly evident at postinfection day 7, revealing increased cell numbers with an outward current component. Furthermore, RMP was inversely modified in A1 and A2 astrocytes during neuroinflammation, and resting Gi was increased from 21 to 30 nS in the latter. In contrast, gap junction communication was significantly decreased in all astrocyte populations associated with inflamed tissues. Collectively, these findings demonstrate the heterogeneity of striatal astrocyte populations, which experience distinct electrophysiological modifications in response to CNS inflammation.

Original languageEnglish (US)
Pages (from-to)112-123
Number of pages12
JournalJournal of Neurophysiology
Volume108
Issue number1
DOIs
StatePublished - Jul 1 2012

Fingerprint

Corpus Striatum
Astrocytes
varespladib methyl
Membrane Potentials
Central Nervous System
Brain Abscess
Gap Junctions
Glial Fibrillary Acidic Protein
Green Fluorescent Proteins
Abscess
Population
Homeostasis
Cell Count
Communication
Cell Membrane
Inflammation

Keywords

  • Gap junction communication
  • Hemichannels
  • Whole cell patch clamp

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Cite this

Neuroinflammation alters voltage-dependent conductance in striatal astrocytes. / Karpuk, Nikolay; Burkovetskaya, Maria; Kielian, Tammy L.

In: Journal of Neurophysiology, Vol. 108, No. 1, 01.07.2012, p. 112-123.

Research output: Contribution to journalArticle

Karpuk, Nikolay ; Burkovetskaya, Maria ; Kielian, Tammy L. / Neuroinflammation alters voltage-dependent conductance in striatal astrocytes. In: Journal of Neurophysiology. 2012 ; Vol. 108, No. 1. pp. 112-123.
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