Neuroendocrine peptides (NPY, GRP, VIP, somatostatin) from the brain and stomach of the alligator

Yunxia W Lundberg, J. Michael Conlon

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Despite the important position of the reptiles in phylogeny, relatively few regulatory peptides from reptilian species have been characterized structurally. Neuropeptide Y was isolated from the brain of the alligator, Alligator mississippiensis, and vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP), its COOH-terminal decapeptide (GRP-10), and somatostatin-14 were isolated from the alligator stomach. The primary structures of NPY and somatostatin-14 are the same as the corresponding peptides from the human, whereas alligator VIP is identical to chicken VIP. The amino acid sequence of GRP (Ala-Pro-Ala-Pro-Ser-Gly-Gly-Gly-Ser-Ala10-Pro-Leu-Ala-Lys-Ile-Tyr-Pro-Arg-Gly-Ser20-His-Trp-Ala-Val- Gly-His-Leu-Met-NH2) contains an additional residue and six substitutions compared with chicken GRP, but alligator GRP-10 is the same as chicken GRP-10. Bombesin was not detected in the stomach extract. The data confirm that evolutionary pressure to conserve the amino acid sequence of NPY and somatostatin-14 has been very strong but demonstrate that pressure to conserve the complete primary structure of GRP has been less than that for other neuroendocrine peptides. The identity of chicken and alligator VIP is consistent with the known close phylogenetic relationship between crocodilians and birds.

Original languageEnglish (US)
Pages (from-to)573-579
Number of pages7
JournalPeptides
Volume14
Issue number3
DOIs
StatePublished - Jan 1 1993

Fingerprint

Gastrin-Releasing Peptide
Alligators and Crocodiles
Vasoactive Intestinal Peptide
Somatostatin
Brain
Stomach
Peptides
Chickens
Amino Acids
Bombesin
Amino Acid Sequence
Neuropeptide Y
Birds
Pressure
Substitution reactions
Reptiles
Phylogeny
neuromedin C

Keywords

  • Alligator brain
  • Alligator stomach
  • Gastrin-releasing peptide
  • Neuropeptide Y
  • Somatostatin
  • Vasoactive intestinal polypeptide

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Neuroendocrine peptides (NPY, GRP, VIP, somatostatin) from the brain and stomach of the alligator. / Lundberg, Yunxia W; Conlon, J. Michael.

In: Peptides, Vol. 14, No. 3, 01.01.1993, p. 573-579.

Research output: Contribution to journalArticle

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