Neuroautoantibody immunoreactivity in relation to aging and stress in apolipoprotein E-deficient mice

You Zhou, Alan Cheshire, Leigh A. Howell, Donna H. Ryan, Ruth B.S. Harris

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Progressive disruption of both the neuroendocrine and immune systems has been correlated with age-associated pathogenesis in patients with Alzheimer's disease and in mice lacking apolipoprotein E (ApoE). In this study, we examined neuroautoimmune and neuroendocrine activities in relation to aging and stress in ApoE-deficient mice. An elevated level of autoantibodies against brain antigens was found in sera from ApoE-deficient mice compared to that of wild-type mice as early as 7-8 weeks of age. However, there was no significant difference between the two genotypes at this age in the effect of stress on serum corticosterone or autoantibody titers. Higher titers of autoantibodies were observed in ~12-week-old ApoE-deficient mice, especially in those exposed to chronic stress. Based on Western analysis, sera from ApoE-deficient mice showed a strong immunoreactivity with ~78 kDa and ~40 kDa brain abundant polypeptides, ~58 kDa non-brain tissue abundant antigen, and others of ~, 80-82 kDa in both the brain and non-brain tissues. Immunofluorescence confocal microscopy showed that the major cellular components recognized by the autoimmune sera from ApoE-deficient mice were associated with neuronal cell nuclei and fiber-like structures in different regions of the brain, including the frontal cortex, lateral cortex and hippocampus. These results suggest that neuroautoimmunity associated with the aging process and exposure to chronic stress may be involved in early development of neurodegeneration in mice with ApoE-deficiency.

Original languageEnglish (US)
Pages (from-to)173-179
Number of pages7
JournalBrain Research Bulletin
Volume49
Issue number3
DOIs
StatePublished - Jun 1 1999

Fingerprint

Apolipoproteins E
Autoantibodies
Brain
Serum
Antigens
Neurosecretory Systems
Frontal Lobe
Corticosterone
Cell Nucleus
Fluorescence Microscopy
Confocal Microscopy
Immune System
Hippocampus
Alzheimer Disease
Genotype
Peptides

Keywords

  • ApoE-deficient mouse
  • Autoimmune sera
  • Neuroantigens
  • Restraint stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuroautoantibody immunoreactivity in relation to aging and stress in apolipoprotein E-deficient mice. / Zhou, You; Cheshire, Alan; Howell, Leigh A.; Ryan, Donna H.; Harris, Ruth B.S.

In: Brain Research Bulletin, Vol. 49, No. 3, 01.06.1999, p. 173-179.

Research output: Contribution to journalArticle

Zhou, You ; Cheshire, Alan ; Howell, Leigh A. ; Ryan, Donna H. ; Harris, Ruth B.S. / Neuroautoantibody immunoreactivity in relation to aging and stress in apolipoprotein E-deficient mice. In: Brain Research Bulletin. 1999 ; Vol. 49, No. 3. pp. 173-179.
@article{721dbcdbc9544a3c9a2310a4f1eec9c0,
title = "Neuroautoantibody immunoreactivity in relation to aging and stress in apolipoprotein E-deficient mice",
abstract = "Progressive disruption of both the neuroendocrine and immune systems has been correlated with age-associated pathogenesis in patients with Alzheimer's disease and in mice lacking apolipoprotein E (ApoE). In this study, we examined neuroautoimmune and neuroendocrine activities in relation to aging and stress in ApoE-deficient mice. An elevated level of autoantibodies against brain antigens was found in sera from ApoE-deficient mice compared to that of wild-type mice as early as 7-8 weeks of age. However, there was no significant difference between the two genotypes at this age in the effect of stress on serum corticosterone or autoantibody titers. Higher titers of autoantibodies were observed in ~12-week-old ApoE-deficient mice, especially in those exposed to chronic stress. Based on Western analysis, sera from ApoE-deficient mice showed a strong immunoreactivity with ~78 kDa and ~40 kDa brain abundant polypeptides, ~58 kDa non-brain tissue abundant antigen, and others of ~, 80-82 kDa in both the brain and non-brain tissues. Immunofluorescence confocal microscopy showed that the major cellular components recognized by the autoimmune sera from ApoE-deficient mice were associated with neuronal cell nuclei and fiber-like structures in different regions of the brain, including the frontal cortex, lateral cortex and hippocampus. These results suggest that neuroautoimmunity associated with the aging process and exposure to chronic stress may be involved in early development of neurodegeneration in mice with ApoE-deficiency.",
keywords = "ApoE-deficient mouse, Autoimmune sera, Neuroantigens, Restraint stress",
author = "You Zhou and Alan Cheshire and Howell, {Leigh A.} and Ryan, {Donna H.} and Harris, {Ruth B.S.}",
year = "1999",
month = "6",
day = "1",
doi = "10.1016/S0361-9230(99)00052-0",
language = "English (US)",
volume = "49",
pages = "173--179",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Neuroautoantibody immunoreactivity in relation to aging and stress in apolipoprotein E-deficient mice

AU - Zhou, You

AU - Cheshire, Alan

AU - Howell, Leigh A.

AU - Ryan, Donna H.

AU - Harris, Ruth B.S.

PY - 1999/6/1

Y1 - 1999/6/1

N2 - Progressive disruption of both the neuroendocrine and immune systems has been correlated with age-associated pathogenesis in patients with Alzheimer's disease and in mice lacking apolipoprotein E (ApoE). In this study, we examined neuroautoimmune and neuroendocrine activities in relation to aging and stress in ApoE-deficient mice. An elevated level of autoantibodies against brain antigens was found in sera from ApoE-deficient mice compared to that of wild-type mice as early as 7-8 weeks of age. However, there was no significant difference between the two genotypes at this age in the effect of stress on serum corticosterone or autoantibody titers. Higher titers of autoantibodies were observed in ~12-week-old ApoE-deficient mice, especially in those exposed to chronic stress. Based on Western analysis, sera from ApoE-deficient mice showed a strong immunoreactivity with ~78 kDa and ~40 kDa brain abundant polypeptides, ~58 kDa non-brain tissue abundant antigen, and others of ~, 80-82 kDa in both the brain and non-brain tissues. Immunofluorescence confocal microscopy showed that the major cellular components recognized by the autoimmune sera from ApoE-deficient mice were associated with neuronal cell nuclei and fiber-like structures in different regions of the brain, including the frontal cortex, lateral cortex and hippocampus. These results suggest that neuroautoimmunity associated with the aging process and exposure to chronic stress may be involved in early development of neurodegeneration in mice with ApoE-deficiency.

AB - Progressive disruption of both the neuroendocrine and immune systems has been correlated with age-associated pathogenesis in patients with Alzheimer's disease and in mice lacking apolipoprotein E (ApoE). In this study, we examined neuroautoimmune and neuroendocrine activities in relation to aging and stress in ApoE-deficient mice. An elevated level of autoantibodies against brain antigens was found in sera from ApoE-deficient mice compared to that of wild-type mice as early as 7-8 weeks of age. However, there was no significant difference between the two genotypes at this age in the effect of stress on serum corticosterone or autoantibody titers. Higher titers of autoantibodies were observed in ~12-week-old ApoE-deficient mice, especially in those exposed to chronic stress. Based on Western analysis, sera from ApoE-deficient mice showed a strong immunoreactivity with ~78 kDa and ~40 kDa brain abundant polypeptides, ~58 kDa non-brain tissue abundant antigen, and others of ~, 80-82 kDa in both the brain and non-brain tissues. Immunofluorescence confocal microscopy showed that the major cellular components recognized by the autoimmune sera from ApoE-deficient mice were associated with neuronal cell nuclei and fiber-like structures in different regions of the brain, including the frontal cortex, lateral cortex and hippocampus. These results suggest that neuroautoimmunity associated with the aging process and exposure to chronic stress may be involved in early development of neurodegeneration in mice with ApoE-deficiency.

KW - ApoE-deficient mouse

KW - Autoimmune sera

KW - Neuroantigens

KW - Restraint stress

UR - http://www.scopus.com/inward/record.url?scp=0033019725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033019725&partnerID=8YFLogxK

U2 - 10.1016/S0361-9230(99)00052-0

DO - 10.1016/S0361-9230(99)00052-0

M3 - Article

VL - 49

SP - 173

EP - 179

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 3

ER -