Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front

Rebecca McLennan, Linus J. Schumacher, Jason A. Morrison, Jessica M. Teddy, Dennis A. Ridenour, Andrew C. Box, Craig L Semerad, Hua Li, William McDowell, David Kay, Philip K. Maini, Ruth E. Baker, Paul M. Kulesa

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Neural crest (NC) cell migration is crucial to the formation of peripheral tissues during vertebrate development. However, how NC cells respond to different microenvironments to maintain persistence of direction and cohesion in multicellular streams remains unclear. To address this, we profiled eight subregions of a typical cranial NC cell migratory stream. Hierarchical clustering showed significant differences in the expression profiles of the lead three subregions compared with newly emerged cells. Multiplexed imaging of mRNA expression using fluorescent hybridization chain reaction (HCR) quantitatively confirmed the expression profiles of lead cells. Computational modeling predicted that a small fraction of lead cells that detect directional information is optimal for successful stream migration. Single-cell profiling then revealed a unique molecular signature that is consistent and stable over time in a subset of lead cells within the most advanced portion of the migratory front, which we term trailblazers. Model simulations that forced a lead cell behavior in the trailing subpopulation predicted cell bunching near the migratory domain entrance. Misexpression of the trailblazer molecular signature by perturbation of two upstream transcription factors agreed with the in silico prediction and showed alterations to NC cell migration distance and stream shape. These data are the first to characterize the molecular diversity within an NC cell migratory stream and offer insights into how molecular patterns are transduced into cell behaviors.

Original languageEnglish (US)
Pages (from-to)2014-2025
Number of pages12
JournalDevelopment (Cambridge)
Volume142
Issue number11
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Neural Crest
Cell Movement
Computer Simulation
Cluster Analysis
Vertebrates
Transcription Factors
Messenger RNA

Keywords

  • Cell migration
  • Chicken
  • Mathematical modeling
  • Molecular profiling
  • Neural crest
  • Numerical simulation
  • Single cell

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

McLennan, R., Schumacher, L. J., Morrison, J. A., Teddy, J. M., Ridenour, D. A., Box, A. C., ... Kulesa, P. M. (2015). Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front. Development (Cambridge), 142(11), 2014-2025. https://doi.org/10.1242/dev.117507

Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front. / McLennan, Rebecca; Schumacher, Linus J.; Morrison, Jason A.; Teddy, Jessica M.; Ridenour, Dennis A.; Box, Andrew C.; Semerad, Craig L; Li, Hua; McDowell, William; Kay, David; Maini, Philip K.; Baker, Ruth E.; Kulesa, Paul M.

In: Development (Cambridge), Vol. 142, No. 11, 2015, p. 2014-2025.

Research output: Contribution to journalArticle

McLennan, R, Schumacher, LJ, Morrison, JA, Teddy, JM, Ridenour, DA, Box, AC, Semerad, CL, Li, H, McDowell, W, Kay, D, Maini, PK, Baker, RE & Kulesa, PM 2015, 'Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front' Development (Cambridge), vol. 142, no. 11, pp. 2014-2025. https://doi.org/10.1242/dev.117507
McLennan, Rebecca ; Schumacher, Linus J. ; Morrison, Jason A. ; Teddy, Jessica M. ; Ridenour, Dennis A. ; Box, Andrew C. ; Semerad, Craig L ; Li, Hua ; McDowell, William ; Kay, David ; Maini, Philip K. ; Baker, Ruth E. ; Kulesa, Paul M. / Neural crest migration is driven by a few trailblazer cells with a unique molecular signature narrowly confined to the invasive front. In: Development (Cambridge). 2015 ; Vol. 142, No. 11. pp. 2014-2025.
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