Neural circuitry of the kidney: NO-containing neurons

M. L. Weiss, S. I. Chowdhury, K. P. Patel, M. J. Kenney, J. Huang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The neurons synthesizing nitric oxide (NO) that are part of the renal sympathetic pathways were located by double-staining for the neuronal isoform of nitric oxide synthase (nNOS) using immunocytochemistry to identify NO-synthesizing neurons and transneuronal tracing following infection of the left kidney with pseudorabies virus (PRV). Following kidney injection with PRV, the animals survived 4-day post-inoculation prior to sacrifice and tissue processing. PRV-infected neurons that double-stained for nNOS were found in the paraventricular hypothalamic nucleus (PVN), the raphe obscurus nucleus (ROb), the ventromedial medulla (VMM), the rostral ventrolateral medulla (rVLM) and the A5 cell group. In the thoracolumbar spinal cord, nNOS neurons co-localized with PRV-infected cells in the dorsal horn in laminae I, III-V ipsilateral to the injected kidney and in lamina X, the intermediolateral cell column, the lateral funiculus, the intercalated nucleus and the central autonomic area. We conclude that NO synthesizing cells may significantly affect renal autonomic pathways in the rat by interacting with the renal sensory and sympathomotor circuitry at multiple sites.

Original languageEnglish (US)
Pages (from-to)269-282
Number of pages14
JournalBrain Research
Volume919
Issue number2
DOIs
StatePublished - Nov 23 2001

Fingerprint

Suid Herpesvirus 1
Nitric Oxide
Kidney
Neurons
Nitric Oxide Synthase Type I
Protein Isoforms
Posterior Horn Cells
Autonomic Pathways
Raphe Nuclei
Paraventricular Hypothalamic Nucleus
Spinal Cord
Immunohistochemistry
Staining and Labeling
Injections
Infection

Keywords

  • Herpesvirus
  • Immunocytochemistry
  • Neuronal isoform of nitric oxide synthase
  • Sprague-Dawley rat
  • Sympathetic motor pathway
  • Transneuronal transport

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Weiss, M. L., Chowdhury, S. I., Patel, K. P., Kenney, M. J., & Huang, J. (2001). Neural circuitry of the kidney: NO-containing neurons. Brain Research, 919(2), 269-282. https://doi.org/10.1016/S0006-8993(01)03030-X

Neural circuitry of the kidney : NO-containing neurons. / Weiss, M. L.; Chowdhury, S. I.; Patel, K. P.; Kenney, M. J.; Huang, J.

In: Brain Research, Vol. 919, No. 2, 23.11.2001, p. 269-282.

Research output: Contribution to journalArticle

Weiss, ML, Chowdhury, SI, Patel, KP, Kenney, MJ & Huang, J 2001, 'Neural circuitry of the kidney: NO-containing neurons', Brain Research, vol. 919, no. 2, pp. 269-282. https://doi.org/10.1016/S0006-8993(01)03030-X
Weiss, M. L. ; Chowdhury, S. I. ; Patel, K. P. ; Kenney, M. J. ; Huang, J. / Neural circuitry of the kidney : NO-containing neurons. In: Brain Research. 2001 ; Vol. 919, No. 2. pp. 269-282.
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